Estrada Dora A, Grella Maicon D, Thyssen Patricia J, Linhares Arício X
Depto. de Parasitologia, Instituto de Biologia, Univ. Estadual de Campinas, Campinas, SP.
Neotrop Entomol. 2009 Mar-Apr;38(2):203-7. doi: 10.1590/s1519-566x2009000200006.
Forensic entomology uses biological and ecological aspects of necrophagous insects to help in criminal investigations to estimate the post-mortem interval (PMI) or to determine the cause of death. Recent papers demonstrated that the presence of toxins in decomposing tissues may alter the insect developmental rate of insects exploiting such tissues as food. Thus, preliminary tests with artificial diets in laboratory are necessary to create a database to investigate and quantify the modifications that can occur with the collected insects from a criminal scene, avoiding any errors on the PMI estimates. The present study aimed to evaluate the developmental rate of Chrysomya albiceps (Wiedemann) reared on: a) artificial diets containing animal tissues: bovine liver (D1), raw muscle (D2), stomach (D3), and chicken heart (D4); b) artificial diet without animal tissue (D5); and c) a control group (C), which had only meat. The efficiency of each substrate was assessed by immature weight gain (mg), larval developmental time, larval and pupal survival, emergence interval and adult size. D1 to D4 diets did not restrict C. albiceps development; however, larvae reared on D1 and D2 diets presented a lower adult emergence rate. D3 and control group showed similarities regarding the efficiency parameters (rate and emergence interval). Thus, the use of diet D3, artificial diet with stomach, is the most recommended.
法医昆虫学利用食尸性昆虫的生物学和生态学特征协助刑事调查,以估计死后间隔时间(PMI)或确定死因。近期论文表明,腐烂组织中的毒素可能会改变以这些组织为食的昆虫的发育速率。因此,有必要在实验室中使用人工饲料进行初步试验,以建立一个数据库,用于调查和量化从犯罪现场采集的昆虫可能出现的变化,避免在PMI估计上出现任何误差。本研究旨在评估在以下饲料上饲养的白纹伊蚊(Wiedemann)的发育速率:a)含有动物组织的人工饲料:牛肝(D1)、生肌肉(D2)、胃(D3)和鸡心(D4);b)不含动物组织的人工饲料(D5);以及c)仅喂食肉类的对照组(C)。通过幼虫体重增加(mg)、幼虫发育时间、幼虫和蛹的存活率、羽化间隔和成虫大小来评估每种饲料的效率。D1至D4饲料并未限制白纹伊蚊的发育;然而,在D1和D2饲料上饲养的幼虫成虫羽化率较低。D3组和对照组在效率参数(速率和羽化间隔)方面表现出相似性。因此,最推荐使用含有胃的人工饲料D3。