Pitchappan R M, Brahmajothi V, Rajaram K, Subramanyam P T, Balakrishnan K, Muthuveeralakshmi R
Unit of Immunogenetics, School of Biological Sciences, Madurai Kamaraj University, India.
Tubercle. 1991 Jun;72(2):133-9. doi: 10.1016/0041-3879(91)90040-y.
In an effort to study the immunological responses to antigens of tubercle bacilli, 49 tuberculin positive and 41 tuberculin negative hospital contacts aged 20-29 years (staff nurses and students working in Government Rajaji Hospital, Madurai, South India) were studied for serum antibodies (IgG, IgM and IgA classes) to BCG by ELISA and diameter of induration to PPD by Mantoux procedures. The two immunological parameters were correlated in regression analysis. The results have revealed higher anti-BCG serum antibody levels in hospital contacts than in non-contacts, significantly higher antibodies in tuberculin negative hospital contacts than in tuberculin positive hospital contacts, an inverse correlation of tuberculin reactivity and antibodies and a bimodal decline (regression) of antibodies against the increase in skin test induration. This study has thus suggested the existence of an immunological spectrum in hospital contacts from south India; persons at one pole of the spectrum were tuberculin negative and possessed significantly elevated antibody levels and those at the other pole of the spectrum were tuberculin positive and possessed low antibody levels. Thus the spectrum of immune reactivity may be due to an inherent susceptibility/resistance of an individual to Mycobacterium tuberculosis.
为了研究对结核杆菌抗原的免疫反应，对49名结核菌素阳性和41名结核菌素阴性的20 - 29岁医院接触者（印度南部马杜赖政府拉贾吉医院工作的护士和学生）进行了研究，通过酶联免疫吸附测定法检测其血清中针对卡介苗的抗体（IgG、IgM和IgA类），并通过芒图氏法检测其对结核菌素纯蛋白衍生物（PPD）的硬结直径。在回归分析中对这两个免疫参数进行了相关性研究。结果显示，医院接触者的抗卡介苗血清抗体水平高于非接触者，结核菌素阴性的医院接触者的抗体水平显著高于结核菌素阳性的医院接触者，结核菌素反应性与抗体呈负相关，且随着皮肤试验硬结增大，抗体呈双峰下降（回归）。因此，这项研究表明印度南部医院接触者中存在免疫谱；免疫谱一端的人结核菌素阴性且抗体水平显著升高，另一端的人结核菌素阳性且抗体水平较低。因此，免疫反应谱可能归因于个体对结核分枝杆菌的固有易感性/抵抗力。
