Cunha Cristina W, Otto Lisa, Taus Naomi S, Knowles Donald P, Li Hong
USDA-ARS, Washington State University, Pullman, 99164-6630, USA.
J Clin Microbiol. 2009 Aug;47(8):2586-9. doi: 10.1128/JCM.00997-09. Epub 2009 Jun 3.
A multiplex real-time PCR was developed using a single pair of primers and fluorescent probes specific for five malignant catarrhal fever viruses and an internal positive control. The assay was able to simultaneously detect and differentiate the viruses in clinical samples with high sensitivity (97.2%) and specificity (100%).
利用一对针对五种恶性卡他热病毒的引物和荧光探针以及一个内部阳性对照,开发了一种多重实时聚合酶链反应。该检测方法能够以高灵敏度(97.2%)和特异性(100%)同时检测和区分临床样本中的病毒。