Odijk M, Baumann A, Lohmann W, van den Brink F T G, Olthuis W, Karst U, van den Berg A
BIOS - the Lab-on-a-Chip group, MESA+ Institute of Nanotechnology, University of Twente, 7500 AE, Enschede, The Netherlands.
Lab Chip. 2009 Jun 21;9(12):1687-93. doi: 10.1039/b822962g. Epub 2009 Mar 23.
We have designed a microfluidic microreactor chip for electrochemical conversion of analytes, containing a palladium reference electrode and platinum working and counter electrodes. The counter electrode is placed in a separate side-channel on chip to prevent unwanted side-products appearing in the measured spectrograms. Using this chip, cyclic voltammograms are measured in volumes of 9.6 nL. Furthermore the conversion efficiency of ferricyanide is characterized using UV/vis-spectroscopy. We have obtained an on-line conversion of 97% using a flow rate of 1 microL/min. We have used the microreactor chip to study the electrochemical metabolism pathway of amodiaquine using electrochemistry (EC)-liquid chromatography (LC)-mass spectrometry (MS). We have compared our results with measurements obtained with commercially available electrochemical flow-through cells. Using our chip it was possible to obtain similar results. Therefore, we have fabricated an electrochemical cell on-chip which is used successfully in EC-UV/vis and EC-LC-MS experiments.
我们设计了一种用于分析物电化学转换的微流控微反应器芯片，其中包含一个钯参比电极以及铂工作电极和对电极。对电极置于芯片上一个单独的侧通道中，以防止在测量的光谱图中出现不需要的副产物。使用该芯片，可在9.6纳升的体积中测量循环伏安图。此外，使用紫外/可见光谱对铁氰化物的转换效率进行了表征。我们以1微升/分钟的流速实现了97%的在线转换。我们已使用该微反应器芯片，通过电化学（EC）-液相色谱（LC）-质谱（MS）研究了阿莫地喹的电化学代谢途径。我们将我们的结果与使用市售电化学流通池获得的测量结果进行了比较。使用我们的芯片能够获得相似的结果。因此，我们制造了一种片上电化学池，并成功地将其用于EC-UV/vis和EC-LC-MS实验。
