BIOS--the Lab-on-a-Chip group, MESA+ Institute of Nanotechnology, University of Twente, P.O. box 217, 7500 AE Enschede, The Netherlands.
Biosens Bioelectron. 2010 Dec 15;26(4):1521-7. doi: 10.1016/j.bios.2010.07.102. Epub 2010 Aug 3.
We have designed an integrated 3-electrode electrochemical cell on-chip with high analyte conversion rates for use in drug metabolism studies. The electrochemical cell contains platinum working and counter electrodes and an iridium oxide pseudo-reference electrode. The pseudo-reference electrode has a pH sensitivity of -52 mV/s, and thus will provide a constant potential in solutions with known and constant pH. The average drift of the iridium oxide electrode is below 5 mV for a typical 15 min conversion experiment. We have been able to mimic the oxidative drug metabolism reactions catalysed by enzymes of the cytochrome P-450 family, normally occurring in the human body. With the chip, the different reaction products of both rat liver cell microsome and human liver cell microsome incubations have been observed.
我们设计了一种集成的 3 电极电化学芯片，具有高分析物转化率，可用于药物代谢研究。该电化学池包含铂工作电极和对电极以及氧化铱伪参比电极。伪参比电极的 pH 值灵敏度为-52 mV/s，因此将在具有已知和恒定 pH 值的溶液中提供恒定的电势。在典型的 15 分钟转化实验中，氧化铱电极的平均漂移低于 5 mV。我们已经能够模拟通常发生在人体内的细胞色素 P-450 家族酶催化的氧化药物代谢反应。使用该芯片，已经观察到大鼠肝微粒体和人肝微粒体孵育的不同反应产物。
