Benninger Richard, Hofmann Oliver, McGinty James, Requejo-Isidro Jose, Munro Ian, Neil Mark, Demello Andrew, French Paul
Opt Express. 2005 Aug 8;13(16):6275-85. doi: 10.1364/opex.13.006275.
We present the application of wide-field time-resolved fluorescence imaging methods for the study of solvent interactions and mixing in microfluidic devices. Time-resolved imaging of fluorescence polarization anisotropy allows us to image the local viscosity of fluorescence in three dimensions in order to directly monitor solvent mixing within a microfluidic channel. This provides a viscosity image acquisition time of the order of minutes, and has been applied to a steady-state laminar flow configuration. To image dynamic fluid mixing in real-time, we demonstrate high-speed fluorescence lifetime imaging at 12.3 Hz applied to DASPI, which directly exhibits a solvent viscosity-dependant fluorescence lifetime. These two methods facilitate a high degree of quantification of microfluidic flow in 3-D and/or at high speed, providing a tool for studying fluid dynamics and for developing enhanced microfluidic assays.
我们展示了宽场时间分辨荧光成像方法在研究微流控装置中溶剂相互作用和混合方面的应用。荧光偏振各向异性的时间分辨成像使我们能够在三维空间中对荧光的局部粘度进行成像，以便直接监测微流控通道内的溶剂混合情况。这提供了大约几分钟的粘度图像采集时间，并已应用于稳态层流配置。为了实时成像动态流体混合，我们展示了以12.3 Hz应用于DASPI的高速荧光寿命成像，其直接表现出与溶剂粘度相关的荧光寿命。这两种方法有助于在三维和/或高速下对微流控流动进行高度量化，为研究流体动力学和开发增强型微流控分析提供了一种工具。
