Grieve Kate, Dubois Arnaud, Simonutti Manuel, Paques Michel, Sahel José, Le Gargasson Jean-François, Boccara Claude
Opt Express. 2005 Aug 8;13(16):6286-95. doi: 10.1364/opex.13.006286.
We present a new high speed full-field optical coherence tomography (OCT) instrument, the first full-field OCT system that is capable of in vivo ocular imaging. An isotropic resolution of ~ 1 mum is achieved thanks to the use of a xenon arc lamp source and relatively high numerical aperture microscope objectives in a Linnik-type interferometer. Full-field illumination allows the capture of two-dimensional en face images in parallel, using a fast CMOS camera as detector array. Each en face image is acquired in a 4 ms period, at a maximum repetition rate of 250 Hz. Detection sensitivity per en face image is 71 dB. Higher sensitivity can be achieved by image correlation and averaging, although frame rate is reduced. We present the first preliminary results of in vivo imaging in the anterior segment of the rat eye, which reveal some cellular features in the corneal layers.
我们展示了一种新型高速全场光学相干断层扫描（OCT）仪器，这是首个能够进行眼部活体成像的全场OCT系统。由于在Linnik型干涉仪中使用了氙弧灯光源和相对较高数值孔径的显微镜物镜，实现了约1微米的各向同性分辨率。全场照明允许使用快速CMOS相机作为探测器阵列并行捕获二维正面图像。每个正面图像在4毫秒内采集，最大重复率为250赫兹。每个正面图像的检测灵敏度为71分贝。通过图像相关和平均可以实现更高的灵敏度，不过帧率会降低。我们展示了大鼠眼前节活体成像的首批初步结果，这些结果揭示了角膜层中的一些细胞特征。
