Wei Bo, Cai Tao, Zhang Rongzhi, Li Aili, Huo Naxin, Li Shan, Gu Yong Q, Vogel John, Jia Jizeng, Qi Yijun, Mao Long
National Key Facility for Crop Gene Resources and Genetic Improvement and Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing, Pr China.
Funct Integr Genomics. 2009 Nov;9(4):499-511. doi: 10.1007/s10142-009-0128-9. Epub 2009 Jun 5.
The small RNA transcriptomes of bread wheat and its emerging model Brachypodium distachyon were obtained by using deep sequencing technology. Small RNA compositions were analyzed in these two species. In addition to 70 conserved microRNAs (miRNAs) from 25 families, 23 novel wheat miRNAs were identified. For Brachypodium, 12 putative miRNAs were predicted from a limited number of expressed sequence tags, of which one was a potential novel miRNA. Also, 94 conserved miRNAs from 28 families were identified in this species. Expression validation was performed for several novel wheat miRNAs. RNA ligase-mediated 5' rapid amplification of complementary DNA ends experiments demonstrated their capability to cleave predicted target genes including three disease-resistant gene analogs. Differential expression of miRNAs was observed between Brachypodium vegetative and reproductive tissues, suggesting their different roles at the two growth stages. Our work significantly increases the novel miRNA numbers in wheat and provides the first set of small RNAs in B. distachyon.
利用深度测序技术获得了普通小麦及其新兴模式植物二穗短柄草的小RNA转录组。分析了这两个物种中的小RNA组成。除了来自25个家族的70个保守微RNA（miRNA）外，还鉴定出23个新的小麦miRNA。对于二穗短柄草，从有限数量的表达序列标签中预测出12个假定的miRNA，其中一个是潜在的新miRNA。此外，在该物种中还鉴定出了来自28个家族的94个保守miRNA。对几个新的小麦miRNA进行了表达验证。RNA连接酶介导的5'互补DNA末端快速扩增实验证明了它们切割预测靶基因的能力，其中包括三个抗病基因类似物。在二穗短柄草的营养组织和生殖组织之间观察到miRNA的差异表达，表明它们在两个生长阶段发挥不同的作用。我们的工作显著增加了小麦中新miRNA的数量，并提供了二穗短柄草的第一套小RNA。
