Hýsek J, Fisar Z, Zizka Z, Kofronova O, Binek B
Institute of Landscape Ecology, Czechoslovak Academy of Sciences, Prague.
Zentralbl Mikrobiol. 1991;146(6):435-43.
Samples of aerosol from the surrounding air were collected by forcing them to impact onto a solid nutrient medium, onto membrane filters, or onto microscope slides on microcover slips. The samples were cultivated or viewed in a fluorescence microscope by using a technique developed by us, or investigated by scanning electron microscopy. The amounts of microorganisms found by cultivation method, i.e. those forming microcolonies (CFU), were on average 85% lower than the amounts determined by the fluorescence technique. Cultivation of microorganisms trapped on Synpor filters of various pore size resulted in lower counts than conventional cultivation. Among bacteria, the genera Micrococcus, Bacillus and Corynebacterium predominated; the genera Neisseria, Actinomyces, Pseudomonas and others were also found. Of micromycetes, the genera Penicillium, Aspergillus and Cladosporium occurred most frequently in air. In the summer season, yeasts and other micromycetes prevailed, whereas in the winter season the bacteria counts were higher. The total counts of all components of the aeroplankton were higher in summer than in winter. Dust particles bounded mainly bacteria.
通过迫使周围空气中的气溶胶撞击固体营养培养基、膜过滤器或载有盖玻片的显微镜载玻片来收集样本。使用我们开发的技术在荧光显微镜下培养或观察样本，或通过扫描电子显微镜进行研究。通过培养方法（即形成微菌落的那些微生物，CFU）发现的微生物数量平均比荧光技术测定的数量低85%。在各种孔径的Synpor过滤器上捕获的微生物培养得到的数量比传统培养法少。在细菌中，微球菌属、芽孢杆菌属和棒状杆菌属占主导；也发现了奈瑟菌属、放线菌属、假单胞菌属等。在微真菌中，青霉属、曲霉属和枝孢属在空气中出现最为频繁。在夏季，酵母菌和其他微真菌占优势，而在冬季细菌数量更高。浮游生物所有成分的总数夏季高于冬季。尘埃颗粒主要附着细菌。
