Wang Hao, Zhang Yun Zeng, Man Chao Xin, Chen Wen Feng, Sui Xin Hua, Li Ying, Zhang Xiao Xia, Chen Wen Xin
Department of Microbiology and Immunology, China Agricultural University, Beijing 100193, People's Republic of China.
Int J Syst Evol Microbiol. 2009 Nov;59(Pt 11):2854-6. doi: 10.1099/ijs.0.010447-0. Epub 2009 Jul 23.
An orange-coloured bacterium, CCBAU 05354(T), was isolated from the soybean rhizosphere following growth on NA medium. The sample was taken from a field in Hebei province, People's Republic of China. The cells were aerobic, Gram-stain-negative, short rods (0.4-0.6 x 0.7-1.7 microm) and non-motile. Growth occurred at 28 degrees C (not at 10 or 37 degrees C), pH 6.0-10.0, and in the presence of 0-1 % NaCl (w/v). Flexirubin pigment was produced and the cells were resistant to some antibiotics. A phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CCBAU 05354(T) was closely related to Niabella soli KACC 12604(T) (95.1 % sequence similarity). The predominant cellular fatty acids were iso-C(15 : 0), iso-C(15 : 1) G, iso-C(17 : 0) 3-OH and summed feature 3 (comprising C(16 : 1)omega7c and/or C(16 : 1)omega6c). The DNA G+C content was 42 mol%. On the basis of the phylogenetic, phenotypic and chemotaxonomic data, strain CCBAU 05354(T) represents a novel species of the genus Niabella, for which the name Niabella yanshanensis sp. nov. is proposed. The type strain is CCBAU 05354(T) (=LMG 24661(T) =HAMBI 3031(T)).
从河北省一块田地的大豆根际分离出一株橙色细菌CCBAU 05354(T)，该样品在NA培养基上生长。细胞为需氧型，革兰氏染色阴性，短杆状（0.4 - 0.6×0.7 - 1.7微米），无运动性。在28℃（10℃或37℃不生长）、pH 6.0 - 10.0以及0 - 1% NaCl（w/v）存在的条件下生长。产生了柔韧霉素色素，细胞对某些抗生素具有抗性。基于16S rRNA基因序列的系统发育分析表明，菌株CCBAU 05354(T)与Niabella soli KACC 12604(T)密切相关（序列相似性为95.1%）。主要的细胞脂肪酸为异-C(15 : 0)、异-C(15 : 1)G、异-C(17 : 0) 3-OH以及总和特征3（包含C(16 : 1)ω7c和/或C(16 : 1)ω6c）。DNA G+C含量为42摩尔%。基于系统发育、表型和化学分类学数据，菌株CCBAU 05354(T)代表Niabella属的一个新物种，为此提出新名称Niabella yanshanensis sp. nov.。模式菌株为CCBAU 05354(T)（=LMG 24661(T) =HAMBI 3031(T)）。
