Department of Microbiology, University of Massachusetts, N418 Morrill IV North; 639 N. Pleasant St., Amherst, MA, 01003, USA.
Department of Geological Sciences, University of Colorado, Boulder, CO, 80309, USA.
Extremophiles. 2022 Feb 21;26(1):13. doi: 10.1007/s00792-022-01263-2.
A deep-sea thermophilic bacterium, strain Ax17, was isolated from 25 °C hydrothermal fluid at Axial Seamount. It was obligately anaerobic and autotrophic, oxidized molecular hydrogen and formate, and reduced synthetic nanophase Fe(III) (oxyhydr)oxide minerals, sulfate, sulfite, thiosulfate, and elemental sulfur for growth. It produced up to 20 mM Fe when grown on ferrihydrite but < 5 mM Fe when grown on akaganéite, lepidocrocite, hematite, and goethite. It was a straight to curved rod that grew at temperatures ranging from 35 to 70 °C (optimum 65 °C) and a minimum doubling time of 7.1 h, in the presence of 1.5-6% NaCl (optimum 3%) and pH 5-9 (optimum 8.0). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain was 90-92% identical to other genera of the family Desulfonauticaceae in the phylum Pseudomonadota. The genome of Ax17 was sequenced, which yielded 2,585,834 bp and contained 2407 protein-coding sequences. Based on overall genome relatedness index analyses and its unique phenotypic characteristics, strain Ax17 is suggested to represent a novel genus and species, for which the name Desulfovulcanus ferrireducens is proposed. The type strain is Ax17 (= DSM 111878 = ATCC TSD-233).
从轴向海山 25°C 热液中分离到一株深海嗜热菌，菌株 Ax17。它是严格厌氧菌和自养菌，能够氧化分子氢和甲酸盐，并还原合成纳米相 Fe(III)（oxyhydr）氧化物矿物、硫酸盐、亚硫酸盐、硫代硫酸盐和元素硫用于生长。当在水铁矿上生长时，它最多可产生 20mM 的 Fe，但在纤铁矿、针铁矿、赤铁矿和针铁矿上生长时，Fe 的产量<5mM。它是一种直的到弯曲的棒状菌，在 35 到 70°C 的温度范围内生长（最佳温度为 65°C），最小倍增时间为 7.1 小时，在 1.5-6%NaCl（最佳 3%）和 pH 5-9（最佳 8.0）的存在下。基于 16S rRNA 基因序列的系统发育分析表明，该菌株与假单胞菌门的脱硫单胞菌科的其他属具有 90-92%的同源性。Ax17 的基因组被测序，共获得 2585834bp，包含 2407 个蛋白质编码序列。基于全基因组相关指数分析及其独特的表型特征，建议菌株 Ax17 代表一个新的属和种，建议命名为脱硫还原铁杆菌。模式菌株为 Ax17（=DSM 111878=ATCC TSD-233）。
