Lämmler C
Institut für Bakteriologie und Immunologie Justus-Liebig-Universität Giessen, F. R. Germany.
Zentralbl Veterinarmed B. 1991 Aug;38(6):468-72. doi: 10.1111/j.1439-0450.1991.tb00897.x.
Staphylococcus chromogenes and Staphylococcus hyicus showed bacteriolytic activities towards a Micrococcus luteus reference strain. This was demonstrated on tryptone soya agar containing M. luteus cells. Both bacteriolytic enzymes could be isolated by ionic exchange chromatography and subsequent gel filtration. The isolated bacteriolysin of S. chromogenes lysed the M. luteus reference culture, was heat inactivated by 95 degrees C and precipitated specifically with antiserum produced against the bacteriolysin of S. hyicus.
产色葡萄球菌和猪葡萄球菌对藤黄微球菌参考菌株表现出溶菌活性。这在含有藤黄微球菌细胞的胰蛋白胨大豆琼脂上得到了证实。两种溶菌酶都可以通过离子交换色谱和随后的凝胶过滤进行分离。产色葡萄球菌分离出的溶菌素可裂解藤黄微球菌参考培养物,在95℃下加热失活,并与针对猪葡萄球菌溶菌素产生的抗血清特异性沉淀。