Frede C, Christ D, Lämmler C
Institut für Bakteriologie und Immunologie, Justus-Liebig-Universität, Giessen.
Zentralbl Bakteriol. 1989 May;271(1):54-60. doi: 10.1016/s0934-8840(89)80053-2.
The LE IIIb fraction of the bacteriolytic enzyme from Staphylococcus hyicus could be isolated by ionic exchange chromatography and subsequent gel filtration. Isoelectric focusing of the highly purified enzyme preparation revealed an isoelectric point at pH 10.3. The lytic activity of LE IIIb on streptococci of various serogroups could be effectively analyzed with the help of an aggregometer and expressed as increase of transmittance at 546 nm. The streptococci differed in their LE IIIb lysis pattern, which was more pronounced with those of serological groups C and D and Streptococcus uberis. The lytic effect of the LE IIIb fraction was optimal at low molarity (0.01 mol/l) of the lysis buffer and reduced in the presence of HgCl2 and EDTA.
来自猪葡萄球菌的溶菌酶的LE IIIb组分可通过离子交换色谱和随后的凝胶过滤进行分离。对高度纯化的酶制剂进行等电聚焦显示其等电点为pH 10.3。借助凝聚仪可有效分析LE IIIb对各种血清群链球菌的裂解活性，并表示为546 nm处透光率的增加。不同血清群的链球菌在LE IIIb裂解模式上存在差异，血清群C和D以及乳房链球菌的差异更为明显。LE IIIb组分的裂解作用在低摩尔浓度（0.01 mol/l）的裂解缓冲液中最佳，在HgCl2和EDTA存在时会降低。
