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用于荧光寿命成像（FLIM）的微型侧视成像探头：用荧光染料、组织结构蛋白和组织标本进行验证

Miniaturized side-viewing imaging probe for fluorescence lifetime imaging (FLIM): validation with fluorescence dyes, tissue structural proteins and tissue specimens.

作者信息

Elson D S, Jo J A, Marcu L

机构信息

Institute of Biomedical Engineering and Department of Biosurgery and Surgical Technology, Imperial College London, Exhibition Road, London SW7 2AZ, UK.

出版信息

New J Phys. 2007;9:127. doi: 10.1088/1367-2630/9/5/127.

DOI:10.1088/1367-2630/9/5/127
PMID:19503759
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2691608/
Abstract

We report a side viewing fibre-based endoscope that is compatible with intravascular imaging and fluorescence lifetime imaging microscopy (FLIM). The instrument has been validated through testing with fluorescent dyes and collagen and elastin powders using the Laguerre expansion deconvolution technique to calculate the fluorescence lifetimes. The instrument has also been tested on freshly excised unstained animal vascular tissues.

摘要

我们报告了一种基于光纤的侧视内窥镜，它与血管内成像和荧光寿命成像显微镜（FLIM）兼容。该仪器已通过使用拉盖尔展开反卷积技术对荧光染料、胶原蛋白和弹性蛋白粉末进行测试来计算荧光寿命，从而得到验证。该仪器还在新鲜切除的未染色动物血管组织上进行了测试。

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Time-domain laser-induced fluorescence spectroscopy apparatus for clinical diagnostics.用于临床诊断的时域激光诱导荧光光谱仪
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Ultrafast Method for the Analysis of Fluorescence Lifetime Imaging Microscopy Data Based on the Laguerre Expansion Technique.基于拉盖尔展开技术的荧光寿命成像显微镜数据的超快分析方法。
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