银杏叶中黄酮类化合物的酶-溶剂提取工艺研究
[Study on enzyme-solvent extraction process of flavonoids from leaves of Ginkgo biloba].
作者信息
Ouyang Na-na, Li Xiang-zhou, Luo Zheng
机构信息
Materials Science and Engineering Institute, Central South University of Forestry and Technology, Changsha 410004, China.
出版信息
Zhong Yao Cai. 2009 Feb;32(2):279-83.
OBJECTIVE
To study the optimum enzyme-solvent extraction conditions of flavonoids from leaves of Ginkgo biloba.
METHODS
To optimize the process by single factor experiments and orthogonal test according to the yield of flavonoids.
RESULTS
The optimum extraction conditions were established as follows: pH 5.7, the concentration of enzyme 0.4 mg/mL, enzymatic hydrolysis temperature 45 degrees C, enzymatic hydrolysis time 2 h, the rate of liquid to material 20:1, extraction temperature 70 degrees C, extraction time 2 h, the concentration of ethanol 70%, the average yield of flavonoids from leaves of Ginkgo biloba was 3.62%.
CONCLUSION
The method is simple and can increase material utility effectively.
目的
研究银杏叶中黄酮类化合物的最佳酶-溶剂提取条件。
方法
根据黄酮类化合物的得率，通过单因素实验和正交试验对工艺进行优化。
结果
确定最佳提取条件如下：pH 5.7，酶浓度0.4 mg/mL，酶解温度45℃，酶解时间2 h，料液比20:1，提取温度70℃，提取时间2 h，乙醇浓度70%，银杏叶中黄酮类化合物的平均得率为3.62%。
结论
该方法简便，能有效提高原料利用率。
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