元宝枫叶片中多酚的提取及其对脂肪酸合酶抑制能力的研究
[Study on extraction of polyphenols from the leaves of Acer truncatum and their inhibitory abilities on fatty acid synthase].
作者信息
Ye Yan-bin, Yang Shuai, Gao Wen, Zhang Feng, Guo Jian-qiang, Zhang Ying-xia
机构信息
College of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Beijing 100069, China.
出版信息
Zhong Yao Cai. 2009 Feb;32(2):283-6.
OBJECTIVE
To optimize a technical process for extracting the polyphenols that can inhibit fatty acid synthase by ethanol from the leaves of Acer truncatum.
METHODS
The extracting time, extracting temperature, extracting solvent and the ratio of solvent to raw material were studied by L16 (4(5)) orthogonal experiments. The extraction rate of polyphenols and the inhibitory efficiency on the fatty acid synthase were utilized as evaluating criteria, and the optimum conditions for the extraction were obtained through summing up the above factors.
RESULTS
The efficient technological conditions were as follows: the concentration of ethanol was 60% (v/v), the ratio of solvent to raw material was 1 g: 30 mL; and the stirring time was 1.5 h at 50 degrees C.
CONCLUSION
The polyphenols that extracted from the leaves of Acer truncatum have inhibitory activity against fatty acid synthase and some of them have stronger inhibitory effect.
目的
优化从元宝枫叶中用乙醇提取可抑制脂肪酸合酶的多酚的工艺过程。
方法
采用L16(4(5))正交试验研究提取时间、提取温度、提取溶剂及溶剂与原料的比例。以多酚提取率和对脂肪酸合酶的抑制效率为评价指标，综合上述因素得出最佳提取条件。
结果
高效工艺条件如下：乙醇浓度为60%（v/v），溶剂与原料的比例为1 g: 30 mL；在50℃下搅拌时间为1.5 h。
结论
从元宝枫叶中提取的多酚对脂肪酸合酶具有抑制活性，其中一些具有较强的抑制作用。
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