Uto Tomofumi, Akagi Takami, Hamasaki Takayuki, Akashi Mitsuru, Baba Masanori
Division of Antiviral Chemotherapy, Center for Chronic Viral Diseases, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan.
Immunol Lett. 2009 Jun 30;125(1):46-52. doi: 10.1016/j.imlet.2009.05.008. Epub 2009 Jun 6.
Vaccine strategy needs efficient adjuvants to induce potent antigen-specific immune responses by targeting antigens to antigen presenting cells followed by their functional maturation. In this study, biodegradable poly(gamma-glutamic acid) (gamma-PGA) nanoparticles (NPs) were examined for their immunological activities in mice. Like lipopolysaccharide, gamma-PGA NPs strongly activated spleen dendritic cells (DCs) and induced their cytokine production and costimulatory molecule expression through the nuclear factor-kappaB and mitogen-activated protein kinase signaling pathways. The immunization of mice with ovalbumin-carrying gamma-PGA NPs could induce the antigen-specific and long-lived effector and central memory CD8(+) T cells as well as antibody responses. Thus, gamma-PGA NPs have great potential as an efficient antigen carrier and strong adjuvant to DCs.
疫苗策略需要高效佐剂，通过将抗原靶向抗原呈递细胞并使其功能成熟，来诱导强大的抗原特异性免疫反应。在本研究中，对可生物降解的聚（γ-谷氨酸）（γ-PGA）纳米颗粒（NPs）在小鼠中的免疫活性进行了检测。与脂多糖一样，γ-PGA NPs强烈激活脾脏树突状细胞（DCs），并通过核因子-κB和丝裂原活化蛋白激酶信号通路诱导其细胞因子产生和共刺激分子表达。用携带卵清蛋白的γ-PGA NPs免疫小鼠可诱导抗原特异性和长寿的效应及中枢记忆CD8(+) T细胞以及抗体反应。因此，γ-PGA NPs作为一种高效的抗原载体和强大的DCs佐剂具有巨大潜力。
