Amrani Souliman, Harnafi Hicham, Gadi Dounia, Mekhfi Hassane, Legssyer Abdelkhaleq, Aziz Mohammed, Martin-Nizard Françoise, Bosca Lisardo
Laboratory of Biochemistry, Faculty of Sciences, University Mohammed I, 60000 Oujda, Morocco.
J Ethnopharmacol. 2009 Aug 17;125(1):157-62. doi: 10.1016/j.jep.2009.05.043. Epub 2009 Jun 6.
In this work the endothelium-dependant vasorelaxant and anti-platelet aggregation activities of an aqueous extract from Ocimum basilicum were studied.
The vasorelaxant effect was undertaken in thoracic aorta from three experimental groups of rats: one of them (NCG) fed with standard diet, the second (HCG) with hypercholesterolemic diet (HCD) and the third (BTG) with hypercholesterolemic diet together with an intragastric administration of Ocimum basilicum extract at a dose of 0.5 g/kg body weight for a period of 10 weeks. The in vitro anti-platelet aggregation of Ocimum basilicum extract was studied using thrombin (0.5 U/ml) and ADP (5 microM) as agonists.
The results show that the HCD statistically decreases vascular relaxation in HCG compared to NCG (p<0.001) and increases the vascular responses to phenylephrine (p<0.02). Ocimum basilicum extract exerts a significant vasorelaxant effect at 10(-5) M (p<0.01) and 10(-4) M carbachol (p=0.001). The plant extract also tends to suppress the elevated contractions induced by HCD (p=0.05). The extract inhibits ADP-induced platelet aggregation by 13%, 28.2%, 30.5%, 44.7% and 53% at a dose of 1, 2, 3, 4 and 5 g/l, respectively. Thrombin-induced platelet activation was also reduced by 15%, 23%, 40%, 38.4%, and 42% at the same doses of extract described above.
The use of Ocimum basilicum as medicinal plant could be beneficial for cardiovascular system.
本研究旨在探讨罗勒水提取物的内皮依赖性血管舒张和抗血小板聚集活性。
在三组实验大鼠的胸主动脉上进行血管舒张作用研究：一组（NCG）喂食标准饮食，第二组（HCG）喂食高胆固醇饮食（HCD），第三组（BTG）喂食高胆固醇饮食并同时胃内给予剂量为0.5 g/kg体重的罗勒提取物，持续10周。使用凝血酶（0.5 U/ml）和ADP（5 microM）作为激动剂，研究罗勒提取物的体外抗血小板聚集作用。
结果显示，与NCG相比，HCD使HCG的血管舒张作用在统计学上显著降低（p<0.001），并增加了对去氧肾上腺素的血管反应（p<0.02）。罗勒提取物在10(-5) M卡巴胆碱时发挥显著的血管舒张作用（p<0.01），在10(-4) M时作用更显著（p=0.001）。该植物提取物还倾向于抑制HCD诱导的收缩增强（p=0.05）。提取物在剂量为1、2、3、4和5 g/l时，分别抑制ADP诱导的血小板聚集13%、28.2%、30.5%、44.7%和53%。在上述相同剂量的提取物作用下，凝血酶诱导的血小板活化也分别降低了15%、23%、40%、38.4%和42%。
将罗勒作为药用植物使用可能对心血管系统有益。
