Department of Pathology, Seoul National University College of Medicine, Seoul, Republic of Korea.
Lung Cancer. 2010 Mar;67(3):301-5. doi: 10.1016/j.lungcan.2009.05.002. Epub 2009 Jun 7.
Fluorescence in situ hybridization (FISH) has been known to be the most representative and standardized test for assessing gene amplification. However, FISH requires a fluorescence microscope, the signals are labile and rapidly fade over time. Recently, chromogenic in situ hybridization (CISH) has emerged as a potential alternative to FISH. The aim of this study is to test the reliability of CISH technique for the detection of epidermal growth factor receptor (EGFR) gene amplification in non-small-cell lung carcinomas (NSCLC), to compare CISH results with FISH. A total of 277 formalin-fixed and paraffin embedded NSCLC tissue samples were retrieved from the surgical pathology archives at Seoul National University Bundang Hospital. CISH and FISH examinations were performed to test EGFR gene amplification status. There was high concordance in the assessment of EGFR gene copy number between CISH and FISH tests (Kappa coefficient=0.83). Excellent concordance was shown between two observers on the interpretation of the CISH results (Kappa coefficient=0.90). In conclusion, CISH result is highly reproducible, accurate and practical method to determine EGFR gene amplification in NSCLC. In addition, CISH allows a concurrent analysis of histological features of the tumors and gene copy numbers.
荧光原位杂交（FISH）已被公认为评估基因扩增最具代表性和标准化的检测方法。然而，FISH 需要荧光显微镜，信号不稳定且随时间迅速衰减。最近，显色原位杂交（CISH）已成为 FISH 的潜在替代方法。本研究旨在测试 CISH 技术检测非小细胞肺癌（NSCLC）中表皮生长因子受体（EGFR）基因扩增的可靠性，并将 CISH 结果与 FISH 进行比较。共从首尔国立大学盆唐医院的外科病理档案中检索了 277 例福尔马林固定和石蜡包埋的 NSCLC 组织样本。进行了 CISH 和 FISH 检查以测试 EGFR 基因扩增状态。CISH 和 FISH 检测在评估 EGFR 基因拷贝数方面具有高度一致性（Kappa 系数=0.83）。两位观察者对 CISH 结果的解释具有极好的一致性（Kappa 系数=0.90）。总之，CISH 是一种高度可重复、准确且实用的方法，可用于确定 NSCLC 中的 EGFR 基因扩增。此外，CISH 允许对肿瘤的组织学特征和基因拷贝数进行同时分析。
