Greenaway Hui Yee, Kurniawan Monica, Price David A, Douek Daniel C, Davenport Miles P, Venturi Vanessa
Complex Systems in Biology Group, Centre for Vascular Research, University of New South Wales, New South Wales, Australia.
Immunol Cell Biol. 2009 Oct;87(7):546-53. doi: 10.1038/icb.2009.38. Epub 2009 Jun 9.
Rhesus macaque models have been instrumental in the development and testing of vaccines before human studies and have provided fundamental insights into the determinants of immune efficacy in a variety of infectious diseases. However, the characterization of antigen-specific T-cell receptor (TCR) repertoires during adaptive immune responses in these models has earlier relied on human TCR gene assignments. Here, we extracted and characterized TCR beta-chain (TRB) genes from the recently sequenced rhesus macaque genome that are homologous to the human TRB genes. Comparison of the rhesus macaque TRB genes with the human TRB genes showed an average best match similarity of 92.9%. Furthermore, we confirmed the usage of most rhesus macaque TRB genes by expressed TCRbeta sequences within epitope-specific TCR repertoires. This primary description of the rhesus macaque TRB genes will provide a standardized nomenclature and enable better characterization of TCR usage in studies that use this species.
恒河猴模型在人类研究之前的疫苗研发和测试中发挥了重要作用，并为多种传染病免疫效力的决定因素提供了基本见解。然而，此前这些模型在适应性免疫反应过程中抗原特异性T细胞受体（TCR）库的表征依赖于人类TCR基因分配。在此，我们从最近测序的恒河猴基因组中提取并鉴定了与人类TRB基因同源的TCRβ链（TRB）基因。恒河猴TRB基因与人类TRB基因的比较显示平均最佳匹配相似度为92.9%。此外，我们通过表位特异性TCR库中表达的TCRβ序列证实了大多数恒河猴TRB基因的使用情况。对恒河猴TRB基因的这一初步描述将提供标准化命名法，并有助于在使用该物种的研究中更好地表征TCR的使用情况。
