正畸引物的体外细胞毒性。

In vitro cytotoxicity of orthodontic primers.

作者信息

D'Antò Vincenzo, Spagnuolo Gianrico, Polito Ilaria, Paduano Sergio, Ambrosio Luigi, Valletta Rosa

机构信息

Institue of Composite and Biomedical Materials - Naples, Italy.

出版信息

Prog Orthod. 2009;10(1):4-11.

PMID:19506741

Abstract

OBJECTIVES

The aim of this study was to evaluate the cytotoxicity of four orthodontic primers: Transbond XT and Transbond MIP (3M, USA), Eagle Fluorsure (American Orthodontics, USA) and Ortho Solo (Ormco, USA).

MATERIALS AND METHODS

Balb 3T3 cells were exposed to different concentrations of primers (0-0.25 mg/ml). Mitochondrial dehydrogenase activity was evaluated by MTT assay and cell necrosis was measured by flow cytometry (propidium iodide staining).

RESULTS

All the materials decreased cell viability in a dose related manner. Cytotoxicity of orthodontic primers based on concentrations which caused a 50% decrease of mitochondrial activity was ranked as follows: Transbond XT (45.57 mg/ml) > Eagle Fluorsure (49.27 mg/ml) > Transbond MIP (64.35 mg/ml) > Ortho solo (70.09 mg/ml).

CONCLUSIONS

Our results suggest that the cytotoxic potencies demonstrated by orthodontic primers might be of clinical relevance since they disturbed cell metabolism and induced cell death in monolayer cultures.

摘要

目的

本研究旨在评估四种正畸粘结剂的细胞毒性：Transbond XT和Transbond MIP（美国3M公司）、Eagle Fluorsure（美国American Orthodontics公司）以及Ortho Solo（美国Ormco公司）。

材料与方法

将Balb 3T3细胞暴露于不同浓度的粘结剂（0 - 0.25毫克/毫升）中。通过MTT法评估线粒体脱氢酶活性，并通过流式细胞术（碘化丙啶染色）测定细胞坏死情况。

结果

所有材料均以剂量相关方式降低细胞活力。基于导致线粒体活性降低50%的浓度，正畸粘结剂的细胞毒性排序如下：Transbond XT（45.57毫克/毫升）> Eagle Fluorsure（49.27毫克/毫升）> Transbond MIP（64.35毫克/毫升）> Ortho solo（70.09毫克/毫升）。