Hahn E C, Ramos L, Kenyon A J
J Natl Cancer Inst. 1977 Jul;59(1):267-71. doi: 10.1093/jnci/59.1.267.
JM-V leukemic lymphoblasts were established in cell culture. The cultured cells (JM-VLC cells) were transplantable in young chicks and produced a disease indistinguishable from JM-V lymphoblastic leukemia as initiated by whole-blood inoculation. JM-VLC cells maintained a normal female karyotype through 13 passages in Rhode Island Red cockerels. With the use of JM-V antisera and antisera from birds with naturally occurring Marek's disease (MD), specific antigens were detected on the surfaces of living cells. Intracellular antigens were detected with anti-MD virus sera after cultivation for at least 1 day at 37 degrees C. In spite of the expression of MD antigens, the presence of herpesvirus particles associated with the cultured cells, and the occurrence of foci of multinucleated cells in kidney cultures from chicks inoculated with cellfree preparations of JM-VLC cells, the pathologic potential of the cultured cells was that of JM-V leukemia.
JM-V白血病淋巴母细胞在细胞培养中得以建立。培养的细胞（JM-VLC细胞）可移植到幼雏体内，并引发一种与通过全血接种引发的JM-V淋巴细胞白血病无法区分的疾病。JM-VLC细胞在罗德岛红公鸡体内传代13次后仍保持正常的雌性核型。利用JM-V抗血清和来自患有自然发生的马立克氏病（MD）的鸟类的抗血清，在活细胞表面检测到了特异性抗原。在37℃培养至少1天后，用抗MD病毒血清检测到细胞内抗原。尽管表达了MD抗原，与培养细胞相关的疱疹病毒颗粒的存在，以及接种JM-VLC细胞无细胞制剂的雏鸡肾脏培养物中出现多核细胞灶，但培养细胞的病理潜能仍为JM-V白血病。
