Calnek B W, Carlisle J C, Fabricant J, Murthy K K, Schat K A
Am J Vet Res. 1979 Apr;40(4):541-8.
An apparently nononcogenic Marek's disease virus (SB-1) and turkey herpesvirus could be readily isolated from spleen, bursa of Fabricius, thymus, and peripheral blood lymphocytes of chickens beginning 4 to 6 days after inoculation, but unlike infections with two isolates of oncogenic Marek's disease virus (JM-10 and CU-2), virus replication in these cells was rare, and necrosis in the organs was essentially absent. Splenic enlargement was observed regularly during the first 4 to 11 days after inoculation, and Marek's disease tumor-associated surface antigen was observed on splenic and other lymphocytes in the four viral inoculation groups. Cellular cytotoxicity of splenic lymphocytes was demonstrated in vitro with cultured Marek's disease tumor cells (MSB-1 lymphoblastoid cell line) as the target in a chromium-release assay. The four viral infections induced sensitized lymphocytes.
接种后4至6天起，可轻易从鸡的脾脏、法氏囊、胸腺和外周血淋巴细胞中分离出一种明显无致癌性的马立克氏病病毒（SB-1）和火鸡疱疹病毒，但与两种致癌性马立克氏病病毒分离株（JM-10和CU-2）感染不同的是，这些细胞中的病毒复制很少见，器官中基本没有坏死现象。接种后最初4至11天期间定期观察到脾脏肿大，并且在四个病毒接种组的脾脏及其他淋巴细胞上观察到马立克氏病肿瘤相关表面抗原。在铬释放试验中，以培养的马立克氏病肿瘤细胞（MSB-1淋巴母细胞系）为靶标，在体外证明了脾脏淋巴细胞的细胞毒性。这四种病毒感染诱导产生了致敏淋巴细胞。
