College of Life Science, Ningxia University, Xixia District, Yinchuan, Ningxia, 750021, China.
Mol Cell Biochem. 2009 Dec;332(1-2):9-16. doi: 10.1007/s11010-009-0168-4. Epub 2009 Jun 9.
MicroRNAs (miRNAs) are a class of small non-coding RNAs found in both animal and plant cells. These approximately 19-26 nucleotide (nt) single-stranded RNAs play role in regulating gene/protein expression by either directly binding to mRNAs and inducing degradation of their target or by translational inhibition of protein expression. In this report, we described the cloning and identification of 22 microRNAs from bovine alveolar macrophage (AM). Eleven of the 22 miRNAs were novel and have not been previously identified in any species, while eight previously unidentified bovine miRNAs were identical to known ortholog miRNAs from human and/or rat (Bta-miR-141, Bta-miR-187, Bta-miR-191, Bta-miR-448, Bta-miR-589, Bta-miR-873, Bta-miR-463, and Bta-miR-562). These results add to the growing database of new miRNAs and suggest new biological functions for miRNAs in AMs. Specially, our data implicates miRNA regulation of antimicrobial targets in alveolar macrophages of bovine lung.
微小 RNA（miRNAs）是一类在动物和植物细胞中发现的小非编码 RNA。这些大约 19-26 个核苷酸（nt）的单链 RNA 通过直接与 mRNAs 结合并诱导其靶标的降解或通过翻译抑制蛋白表达来调节基因/蛋白表达。在本报告中，我们描述了从牛肺泡巨噬细胞（AM）中克隆和鉴定 22 个 microRNAs。22 个 microRNAs 中有 11 个是新的，在任何物种中都没有被先前鉴定过，而 8 个先前未被鉴定的牛 microRNAs 与来自人类和/或大鼠的已知同源 microRNAs 相同（Bta-miR-141、Bta-miR-187、Bta-miR-191、Bta-miR-448、Bta-miR-589、Bta-miR-873、Bta-miR-463 和 Bta-miR-562）。这些结果增加了新 microRNAs 的不断增长的数据库，并暗示了 microRNAs 在 AMs 中的新生物学功能。特别是，我们的数据表明 miRNA 调节牛肺肺泡巨噬细胞中的抗菌靶标。
