枯草芽孢杆菌中的过氧化氢传感：一切都与（金属）调节因子有关。
Hydrogen peroxide sensing in Bacillus subtilis: it is all about the (metallo)regulator.
作者信息
Giedroc David P
机构信息
Department of Chemistry, Indiana University, Bloomington, IN 47405-7002, USA.
出版信息
Mol Microbiol. 2009 Jul;73(1):1-4. doi: 10.1111/j.1365-2958.2009.06752.x. Epub 2009 Jun 8.
Abstract
Microorganisms have evolved an impressive array of mechanisms to adapt to stress induced by reactive oxygen species (ROS) of virtually any kind. One such regulator is Bacillus subtilis PerR, a member of the ubiquitous Fur (Ferric uptake regulator) family of metalloregulatory repressors, which senses hydrogen peroxide. In this issue of Molecular Microbiology, Duarte, Latour and colleagues report the structure of the Mn(II)-bound form of PerR, a first for the Fe/Mn-selective members of the Fur family. The structure reveals how a regulatory metal drives a quaternary structural switch that allosterically activates the PerR dimer to bind its DNA operator, while also providing detailed insight into the mechanism of metal-catalysed ligand oxidation and transcriptional derepression that uniquely characterizes PerR.
摘要
微生物已经进化出一系列令人印象深刻的机制来适应几乎任何种类的活性氧（ROS）所诱导的应激。其中一种调节因子是枯草芽孢杆菌的PerR，它是普遍存在的金属调节阻遏物Fur（铁摄取调节因子）家族的成员之一，可感知过氧化氢。在本期《分子微生物学》中，杜阿尔特、拉图尔及其同事报道了结合锰（II）形式的PerR的结构，这是Fur家族中Fe/Mn选择性成员的首个此类结构。该结构揭示了一种调节金属如何驱动四级结构转换，从而变构激活PerR二聚体以结合其DNA操纵子，同时还深入详细地阐明了金属催化的配体氧化和转录去抑制机制，这是PerR独有的特征。
相似文献
1
Hydrogen peroxide sensing in Bacillus subtilis: it is all about the (metallo)regulator.枯草芽孢杆菌中的过氧化氢传感：一切都与（金属）调节因子有关。
Mol Microbiol. 2009 Jul;73(1):1-4. doi: 10.1111/j.1365-2958.2009.06752.x. Epub 2009 Jun 8.
2
Structural characterization of the active form of PerR: insights into the metal-induced activation of PerR and Fur proteins for DNA binding.过氧化物还原酶（PerR）活性形式的结构表征：深入了解金属诱导的PerR和铁摄取调节蛋白（Fur）对DNA结合的激活作用。
Mol Microbiol. 2009 Jul;73(1):20-31. doi: 10.1111/j.1365-2958.2009.06753.x. Epub 2009 Jun 8.
3
Regulation of the Bacillus subtilis fur and perR genes by PerR: not all members of the PerR regulon are peroxide inducible.PerR对枯草芽孢杆菌fur和perR基因的调控：并非PerR调控子的所有成员都可被过氧化物诱导。
J Bacteriol. 2002 Jun;184(12):3276-86. doi: 10.1128/JB.184.12.3276-3286.2002.
4
Bacillus subtilis Fur Is a Transcriptional Activator for the PerR-Repressed Gene, Encoding an Iron Efflux Pump.枯草芽孢杆菌 Fur 是 PerR 抑制基因的转录激活因子，该基因编码一种铁外排泵。
J Bacteriol. 2020 Mar 26;202(8). doi: 10.1128/JB.00697-19.
5
Roles of metal ions and hydrogen peroxide in modulating the interaction of the Bacillus subtilis PerR peroxide regulon repressor with operator DNA.金属离子和过氧化氢在调节枯草芽孢杆菌PerR过氧化物调节子阻遏物与操纵基因DNA相互作用中的作用
Mol Microbiol. 2001 Aug;41(4):849-59. doi: 10.1046/j.1365-2958.2001.02543.x.
6
The difference in in vivo sensitivity between Bacillus licheniformis PerR and Bacillus subtilis PerR is due to the different cellular environments.地衣芽孢杆菌PerR和枯草芽孢杆菌PerR在体内敏感性上的差异是由于不同的细胞环境所致。
Biochem Biophys Res Commun. 2017 Feb 26;484(1):125-131. doi: 10.1016/j.bbrc.2017.01.060. Epub 2017 Jan 16.
7
The PerR transcription factor senses H2O2 by metal-catalysed histidine oxidation.PerR转录因子通过金属催化的组氨酸氧化来感知过氧化氢。
Nature. 2006 Mar 16;440(7082):363-7. doi: 10.1038/nature04537.
8
The roles of two O-donor ligands in the Fe-binding and HO-sensing by the Fe-dependent HO sensor PerR.两种 O-供体配体在 Fe 依赖性 HO 传感器 PerR 的 Fe 结合和 HO 感应中的作用。
Biochem Biophys Res Commun. 2018 Jun 22;501(2):458-464. doi: 10.1016/j.bbrc.2018.05.012. Epub 2018 May 10.
9
Origins of specificity and cross-talk in metal ion sensing by Bacillus subtilis Fur.枯草芽孢杆菌 Fur 蛋白对金属离子感应的特异性和串扰的起源
Mol Microbiol. 2012 Dec;86(5):1144-55. doi: 10.1111/mmi.12049. Epub 2012 Oct 12.
10
Biochemical characterization of the structural Zn2+ site in the Bacillus subtilis peroxide sensor PerR.枯草芽孢杆菌过氧化物传感器PerR中结构锌离子位点的生化特性
J Biol Chem. 2006 Aug 18;281(33):23567-78. doi: 10.1074/jbc.M603968200. Epub 2006 Jun 8.
引用本文的文献
1
Lon-dependent proteolysis in oxidative stress responses.氧化应激反应中Lon 依赖性蛋白水解作用
J Bacteriol. 2025 Jul 24;207(7):e0000525. doi: 10.1128/jb.00005-25. Epub 2025 Jun 6.
2
Diel investments in metabolite production and consumption in a model microbial system.模型微生物系统中代谢物产生和消耗的昼夜投入。
ISME J. 2022 May;16(5):1306-1317. doi: 10.1038/s41396-021-01172-w. Epub 2021 Dec 17.
3
Bacterial zinc uptake regulator proteins and their regulons.细菌锌摄取调控蛋白及其调控基因。
Biochem Soc Trans. 2018 Aug 20;46(4):983-1001. doi: 10.1042/BST20170228. Epub 2018 Jul 31.
4
Structure and function of the peroxide stress regulator (PerR), an atypical PerR devoid of a structural metal-binding site.过氧化物应激调节剂（PerR）的结构与功能，一种缺乏结构金属结合位点的非典型 PerR。
J Biol Chem. 2018 Jan 12;293(2):497-509. doi: 10.1074/jbc.M117.804443. Epub 2017 Nov 16.
5
Distinct structural features of the peroxide response regulator from group A Streptococcus drive DNA binding.A 组链球菌过氧化物应答调节因子的独特结构特征驱动 DNA 结合。
PLoS One. 2014 Feb 21;9(2):e89027. doi: 10.1371/journal.pone.0089027. eCollection 2014.
6
Prediction of the mechanism of action of fusaricidin on Bacillus subtilis.预测 Fusaricidin 对枯草芽孢杆菌作用机制。
PLoS One. 2012;7(11):e50003. doi: 10.1371/journal.pone.0050003. Epub 2012 Nov 21.
7
ROS homeostasis during development: an evolutionary conserved strategy.发育过程中 ROS 平衡：一种进化保守的策略。
Cell Mol Life Sci. 2012 Oct;69(19):3245-57. doi: 10.1007/s00018-012-1092-4. Epub 2012 Jul 28.
8
Metal site occupancy and allosteric switching in bacterial metal sensor proteins.细菌金属传感器蛋白中的金属位点占据和变构开关。
Arch Biochem Biophys. 2012 Mar 15;519(2):210-22. doi: 10.1016/j.abb.2011.11.021. Epub 2011 Dec 8.
9
Inference of the transcriptional regulatory network in Staphylococcus aureus by integration of experimental and genomics-based evidence.通过整合实验和基于基因组学的证据来推断金黄色葡萄球菌的转录调控网络。
J Bacteriol. 2011 Jul;193(13):3228-40. doi: 10.1128/JB.00350-11. Epub 2011 Apr 29.
10
Metalloregulatory proteins: metal selectivity and allosteric switching.金属调控蛋白：金属选择性和变构开关。
Biophys Chem. 2011 Jul;156(2-3):103-14. doi: 10.1016/j.bpc.2011.03.010. Epub 2011 Apr 5.
本文引用的文献
1
Structural characterization of the active form of PerR: insights into the metal-induced activation of PerR and Fur proteins for DNA binding.过氧化物还原酶（PerR）活性形式的结构表征：深入了解金属诱导的PerR和铁摄取调节蛋白（Fur）对DNA结合的激活作用。
Mol Microbiol. 2009 Jul;73(1):20-31. doi: 10.1111/j.1365-2958.2009.06753.x. Epub 2009 Jun 8.
2
Structural basis for the specialization of Nur, a nickel-specific Fur homolog, in metal sensing and DNA recognition.Nur（一种镍特异性Fur同源蛋白）在金属传感和DNA识别方面特化的结构基础。
Nucleic Acids Res. 2009 Jun;37(10):3442-51. doi: 10.1093/nar/gkp198. Epub 2009 Mar 31.
3
Structural and functional characterization of 2-oxo-histidine in oxidized PerR protein.氧化型PerR蛋白中2-氧代组氨酸的结构与功能表征
Nat Chem Biol. 2009 Jan;5(1):53-9. doi: 10.1038/nchembio.133. Epub 2008 Dec 14.
4
Crystal structure of the [2Fe-2S] oxidative-stress sensor SoxR bound to DNA.与DNA结合的[2Fe-2S]氧化应激传感器SoxR的晶体结构。
Proc Natl Acad Sci U S A. 2008 Mar 18;105(11):4121-6. doi: 10.1073/pnas.0709188105. Epub 2008 Mar 11.
5
Cellular defenses against superoxide and hydrogen peroxide.细胞对超氧化物和过氧化氢的防御机制。
Annu Rev Biochem. 2008;77:755-76. doi: 10.1146/annurev.biochem.77.061606.161055.
6
Nickel-specific response in the transcriptional regulator, Escherichia coli NikR.转录调节因子大肠杆菌镍响应调节蛋白（NikR）中的镍特异性反应
J Am Chem Soc. 2007 Apr 25;129(16):5085-95. doi: 10.1021/ja068505y. Epub 2007 Mar 31.
7
Functional specialization within the Fur family of metalloregulators.金属调节蛋白Fur家族内的功能特化。
Biometals. 2007 Jun;20(3-4):485-99. doi: 10.1007/s10534-006-9070-7. Epub 2007 Jan 10.
8
Crystal structure and function of the zinc uptake regulator FurB from Mycobacterium tuberculosis.结核分枝杆菌锌摄取调节蛋白FurB的晶体结构与功能
J Biol Chem. 2007 Mar 30;282(13):9914-9922. doi: 10.1074/jbc.M609974200. Epub 2007 Jan 9.
9
Crystal structure of the apo-PerR-Zn protein from Bacillus subtilis.来自枯草芽孢杆菌的脱辅基PerR-Zn蛋白的晶体结构。
Mol Microbiol. 2006 Sep;61(5):1211-9. doi: 10.1111/j.1365-2958.2006.05313.x.
10
Biochemical characterization of the structural Zn2+ site in the Bacillus subtilis peroxide sensor PerR.枯草芽孢杆菌过氧化物传感器PerR中结构锌离子位点的生化特性
J Biol Chem. 2006 Aug 18;281(33):23567-78. doi: 10.1074/jbc.M603968200. Epub 2006 Jun 8.
Zotero 插件