Dipartimento di Biologia Animale ed Ecologia Marina, Università di Messina, Messina, Italy.
J Appl Microbiol. 2009 Dec 1;107(6):1935-46. doi: 10.1111/j.1365-2672.2009.04378.x. Epub 2009 May 12.
The aim of the present work was to characterize the heterotrophic bacterial community of a marine recirculating aquaculture system (RAS).
An experimental RAS was sampled for the rearing water (RW) and inside the biofilter. Samples were analysed for bacterial abundances, community structure and composition by using a combination of culture-dependent and -independent techniques. The most represented species detected among biofilter clones was Pseudomonas stutzeri, while Ruegeria spp. and Roseobacter spp. were more abundant among isolates. In comparison, the genera Roseobacter and Ruegeria were well represented in both the biofilter and the RW samples. A variety of possible bacterial pathogens (e.g. Vibrio spp., Erwinia spp. and Coxiella spp.) were also identified in this study.
Results revealed that the bacterial community in the RW was quite different to that associated with the biofilter. Moreover, data obtained suggest that the whole bacterial community can be involved in maintaining an effective and a stable rearing environment (shelter effect).
Improving the reliability and the sustainability of RAS depends on the correct management of the bacterial populations inside it. This study furnishes more accurate information on the bacterial populations and better clarifies the existing relationships between the bacterial flora in the RW and that associated with the biofilter.
本研究旨在描述海水循环水产养殖系统（RAS）中的异养细菌群落特征。
实验性 RAS 用于养殖水（RW）和生物滤池内部的采样。采用培养依赖和非依赖技术的组合，对细菌丰度、群落结构和组成进行分析。在生物滤池克隆中检测到的最具代表性的物种是施氏假单胞菌，而鲁氏菌属和玫瑰杆菌属在分离物中更为丰富。相比之下，玫瑰杆菌属和鲁氏菌属在生物滤池和 RW 样本中都有很好的代表。本研究还鉴定出了多种可能的细菌病原体（例如弧菌属、欧文氏菌属和柯克斯氏体属）。
结果表明，RW 中的细菌群落与生物滤池中的群落有很大不同。此外，获得的数据表明，整个细菌群落可能参与维持有效的稳定养殖环境（庇护效应）。
提高 RAS 的可靠性和可持续性取决于对其内部细菌种群的正确管理。本研究提供了有关细菌种群的更准确信息，并更好地阐明了 RW 中的细菌菌群与生物滤池相关菌群之间的现有关系。
