Department of Dermatology, Dresden-Friedrichstadt, Dresden, Germany.
J Eur Acad Dermatol Venereol. 2009 Dec;23(12):1409-13. doi: 10.1111/j.1468-3083.2009.03323.x. Epub 2009 Jun 5.
Psoriatic lesions are characterized by induration, scaling and erythema. Erythema is a result of inflammation and increased microvascular blood flow. Anthralin is the strongest topical antipsoriatic drug that causes clearing of psoriatic lesions and temporary remission.
The objective evaluation of skin perfusion might be a suitable way to gain a better insight in the pathophysiological process of this disease and to evaluate the response to antipsoriatic anthralin therapy.
We evaluated 21 psoriatic lesions (plaques, patches and pinpoint lesions) including 4 lesions in remission with anthralin induced erythema and 4 controls of healthy, uninvolved skin. We performed the measurements with a combined fluorescence and remission imaging (FRIS). The FRIS sensor is coupled with a touch screen industrial computer. The equipment consists of a white-light halogen lamp (20 W), two VIS-spectrometer modules (Zeiss) for remission detection and references. Imaging is realized by CCD-colour camera module and white light ring-lighting. Fluorescence emission was realized using an ultraviolet LED with a wavelength of 370 nm. The fluorescence detector is a highly sensitive MCS CCD (Zeiss) with an integration time of 2.5 sec.
Spectral remission of psoriatic skin is characterized by a pronounced decrease (60-80%) of the haemoglobin double-peak compared to uninvolved skin. The NADH-fluorescence is diminished in lesional psoriatic skin including anthralin-treated areas with clinical remission.
Vascular perfusion is increased in psoriatic lesions as demonstrated by remission spectroscopy. NADH-fluorescence is reduced in lesional psoriatic skin and in anthralin-induced erythema. FRIS is a suitable tool for objective evaluation of the cutaneous response to antipsoriatic treatment.
银屑病皮损的特征为硬化、脱屑和红斑。红斑是炎症和微脉管血流增加的结果。蒽林是最强的局部抗银屑病药物，可导致银屑病皮损清除和暂时缓解。
皮肤灌注的客观评估可能是更好地了解这种疾病的病理生理过程并评估抗银屑病蒽林治疗反应的合适方法。
我们评估了 21 处银屑病皮损（斑块、斑片和点状皮损），包括 4 处用蒽林诱导红斑处于缓解期的皮损和 4 处健康、未受累皮肤的对照。我们使用荧光和缓解成像（FRIS）联合进行测量。FRIS 传感器与触摸屏工业计算机相连。该设备包括一个 20 W 的卤素白光灯、两个用于缓解检测和参考的 VIS-光谱仪模块（蔡司）。成像通过 CCD 彩色相机模块和白光环形照明实现。荧光发射使用波长为 370nm 的紫外线 LED 实现。荧光探测器是具有 2.5 秒积分时间的高灵敏度 MCS CCD（蔡司）。
银屑病皮肤的光谱缓解表现为与未受累皮肤相比，血红蛋白双峰明显下降（60-80%）。包括用蒽林治疗处于临床缓解期的皮损在内，病变银屑病皮肤中的 NADH 荧光减少。
如缓解光谱所示，银屑病皮损中的血管灌注增加。病变银屑病皮肤和蒽林诱导的红斑中 NADH 荧光减少。FRIS 是客观评估抗银屑病治疗皮肤反应的合适工具。
