Rellán Sandra, Osswald Joana, Saker Martin, Gago-Martinez Ana, Vasconcelos Vitor
Departamento de Química Analítica y Alimentaria, Facultad de Química, Edificio de Ciencias Experimentales, Campus Universitario, Universidad de Vigo, Vigo, Spain.
Food Chem Toxicol. 2009 Sep;47(9):2189-95. doi: 10.1016/j.fct.2009.06.004. Epub 2009 Jun 9.
Anatoxin-a is a potent neurotoxin produced by several species of cyanobacteria. This alkaloid may cause fatal intoxication to exposed organisms and this has raised concerns over the increasing popularity of food supplements containing cyanobacteria. These are being marketed with alleged health properties for animal and human consumption. These supplements most commonly contain the genera Spirulina (Arthrospira) and Aphanizomenon and their consumption represent a potential route for anatoxin-a exposure in cases where adequate quality control is not undertaken. In this work, several dietary supplements containing cyanobacteria from different commercial suppliers were evaluated for the presence of anatoxin-a by high performance liquid chromatography with fluorescence detection. Additionally, the presence of the previously derivatized anatoxin-a was confirmed by using Gas chromatography-mass spectrometry. A total of 39 samples were analysed in the study. Results showed that three of the samples (7.7%) contained anatoxin-a, at concentrations ranging from 2.50 to 33 microg g(-1). Quality control of cyanobacterial food supplements is required to avoid potential health effects in humans and animals.
类毒素-a是由几种蓝藻产生的一种强效神经毒素。这种生物碱可能会对接触到的生物体造成致命中毒，这引发了人们对含有蓝藻的食品补充剂日益流行的担忧。这些补充剂以所谓的对动物和人类消费有益健康的特性进行销售。这些补充剂最常见的含有螺旋藻属（节旋藻属）和水华束丝藻属，在没有进行充分质量控制的情况下，食用这些补充剂是类毒素-a暴露的一个潜在途径。在这项工作中，通过高效液相色谱荧光检测法对来自不同商业供应商的几种含蓝藻的膳食补充剂进行了类毒素-a检测。此外，通过气相色谱-质谱法确认了先前衍生化的类毒素-a的存在。该研究共分析了39个样品。结果显示，其中三个样品（7.7%）含有类毒素-a，浓度范围为2.50至33微克/克。需要对蓝藻食品补充剂进行质量控制，以避免对人类和动物产生潜在的健康影响。
