Osswald Joana, Rellán Sandra, Carvalho António Paulo, Gago Ana, Vasconcelos Vitor
Interdisciplinary Centre of Marine and Environmental Research-CIMAR-Laboratório Associado, Rua dos Bragas, 289, 4050 123 Porto, Portugal.
Toxicon. 2007 Apr;49(5):693-8. doi: 10.1016/j.toxicon.2006.11.010. Epub 2006 Nov 24.
The worldwide increase of eutrophication in fresh water bodies has caused cyanobacterial blooms to be more frequent. Anatoxin-a is a potent neurotoxin known to be produced by several genera of cyanobacteria including Anabaena. In this work, we exposed juvenile carps to freeze-dried cells of a toxic strain of the cyanobacterium Anabaena sp. during a 4-day period. Two different cell density--10(5) and 10(7) cell ml(-1)--were assayed. Lethality and anatoxin-a concentration in the whole fish were determined. In the higher cell density, all fish died between 26 and 29 h after exposure to toxin, whereas in the 10(5) cell ml(-1) density no deaths were observed. Levels of anatoxin-a in the whole fish ranged between 0.031 microg g(-1)d.w. at the 10(5) cell ml(-1) concentration and 0.768 microg g(-1)d.w. at 10(7) cell ml(-1). Minor uptake of anatoxin-a occurred.
全球淡水水体富营养化加剧，导致蓝藻水华更加频繁。类毒素-a是一种强效神经毒素，已知由包括鱼腥藻在内的几种蓝藻产生。在本研究中，我们将幼鲤暴露于蓝藻鱼腥藻有毒菌株的冻干细胞中4天。测定了两种不同的细胞密度——10⁵和10⁷个细胞/毫升。测定了全鱼的致死率和类毒素-a浓度。在较高细胞密度下，所有鱼在接触毒素后26至29小时内死亡，而在10⁵个细胞/毫升的密度下未观察到死亡。全鱼中的类毒素-a水平在10⁵个细胞/毫升浓度下为0.031微克/克干重，在10⁷个细胞/毫升时为0.768微克/克干重。类毒素-a的吸收量较小。
