Ibarz Pavón Ana Belén, Maiden Martin C J
Department of Zoology and Peter Medawar Building for Pathogen Research, University of Oxford, South Parks Road, , Oxford, UK.
Methods Mol Biol. 2009;551:129-40. doi: 10.1007/978-1-60327-999-4_11.
Multilocus sequence typing (MLST) was first proposed in 1998 as a typing approach that enables the unambiguous characterization of bacterial isolates in a standardized, reproducible, and portable manner using the human pathogen Neisseria meningitidis as the exemplar organism. Since then, the approach has been applied to a large and growing number of organisms by public health laboratories and research institutions. MLST data, shared by investigators over the world via the Internet, have been successfully exploited in applications ranging from molecular epidemiological investigations to population biology and evolutionary analyses. This chapter describes the practical steps in the development and application of an MLST scheme and some of the common tools and techniques used to obtain the maximum benefit from the data. Considerations pertinent to the implementation of high-capacity MLST projects (i.e., those involving thousands of isolates) are discussed.
多位点序列分型（MLST）于1998年首次提出，作为一种分型方法，它以人类病原体脑膜炎奈瑟菌作为范例生物，能够以标准化、可重复和便于携带的方式明确鉴定细菌分离株。从那时起，公共卫生实验室和研究机构已将该方法应用于大量且数量不断增加的生物体。世界各地的研究人员通过互联网共享的MLST数据已成功应用于从分子流行病学调查到群体生物学和进化分析等各种应用中。本章描述了开发和应用MLST方案的实际步骤以及为从数据中获取最大收益而使用的一些常用工具和技术。还讨论了与实施高容量MLST项目（即涉及数千个分离株的项目）相关的注意事项。
