Jolley Keith A, Chan Man-Suen, Maiden Martin C J
The Peter Medawar Building for Pathogen Research and Department of Zoology, University of Oxford, OX1 3SY, UK.
BMC Bioinformatics. 2004 Jul 1;5:86. doi: 10.1186/1471-2105-5-86.
Multi-locus sequence typing (MLST) is a method of typing that facilitates the discrimination of microbial isolates by comparing the sequences of housekeeping gene fragments. The mlstdbNet software enables the implementation of distributed web-accessible MLST databases that can be linked widely over the Internet.
The software enables multiple isolate databases to query a single profiles database that contains allelic profile and sequence definitions. This separation enables isolate databases to be established by individual laboratories, each customized to the needs of the particular project and with appropriate access restrictions, while maintaining the benefits of a single definitive source of profile and sequence information. Databases are described by an XML file that is parsed by a Perl CGI script. The software offers a large number of ways to query the databases and to further break down and export the results generated. Additional features can be enabled by installing third-party (freely available) tools.
Development of a distributed structure for MLST databases offers scalability and flexibility, allowing participating centres to maintain ownership of their own data, without introducing duplication and data integrity issues.
多位点序列分型（MLST）是一种分型方法，通过比较管家基因片段的序列来促进微生物分离株的鉴别。mlstdbNet软件能够实现分布式的、可通过网络访问的MLST数据库，这些数据库可以在互联网上广泛链接。
该软件允许多个分离株数据库查询单个包含等位基因谱和序列定义的谱数据库。这种分离使得各个实验室能够建立分离株数据库，每个数据库都根据特定项目的需求进行定制，并设置适当的访问限制，同时保留单一权威的谱和序列信息源所带来的好处。数据库由一个XML文件描述，该文件由一个Perl CGI脚本解析。该软件提供了大量查询数据库以及进一步分解和导出所生成结果的方法。通过安装第三方（免费提供）工具可以启用其他功能。
开发MLST数据库的分布式结构具有可扩展性和灵活性，允许参与的中心保持对其自身数据的所有权，而不会引入重复和数据完整性问题。
