Department of Parasitology, Faculty of Medicine, Aydin Adnan Menderes University, Aydin, 09100, Turkey.
Department of Plant and Animal Production, Aydin Adnan Menderes University, Sultanhisar MYO, Aydin, 09100, Turkey.
Acta Parasitol. 2024 Sep;69(3):1576-1586. doi: 10.1007/s11686-024-00896-x. Epub 2024 Aug 20.
Dientamoeba fragilis (D. fragilis) is a common intestinal protozoan with a global distribution. In the present study, we aimed to determine genetic diversity of D. fragilis isolates with multilocus sequence typing (MLST) in the southwest of Turkey and analyse the clinical findings.
The study included faecal samples from 200 individuals in Aydin, Turkey. The positivity of D. fragilis was determined with 18 S rRNA gene-based PCR assay. Six nested-PCR reactions were set to amplify partial D. fragilis housekeeping genes in the positive samples. The sequences were aligned with the references from GenBank to detect nucleotide polymorphisms and haplotypes. Additionally, the clinical findings and demographic characteristics of patients were statistically analysed between D. fragilis-infected and non-infected cases.
The positivity of D. fragilis was 16% (32 out of 200 cases) with 18 S rRNA based-PCR, and all were classified as "genotype 1". The analysis of six MLST loci revealed different haplotypes only at one locus; the remaining five loci exhibited no polymorphisms. The haplotypes in the present study were identical to at least one previously reported reference, except the locus "large subunit of RNA polymerase II" locus. There were no significant differences in any of the clinical findings or demographic characteristics between the infected and non-infected groups.
Our study revealed a low genetic diversity of D. fragilis isolates from Turkey, like other countries including Italy, Denmark, the UK, Australia, and Brazil. The high degree of sequence similarity in housekeeping genes indicated the clonal distribution of D. fragilis.
脆弱双核阿米巴（Dientamoeba fragilis）是一种分布广泛的常见肠道原生动物。本研究旨在通过多位点序列分型（MLST）确定土耳其西南部脆弱双核阿米巴分离株的遗传多样性，并分析临床发现。
该研究纳入了来自土耳其艾登的 200 名个体的粪便样本。使用基于 18S rRNA 基因的 PCR 检测法确定脆弱双核阿米巴的阳性率。在阳性样本中设置了 6 个嵌套 PCR 反应来扩增脆弱双核阿米巴的部分管家基因。将序列与 GenBank 中的参考序列进行比对，以检测核苷酸多态性和单倍型。此外，还对感染和未感染患者的临床发现和人口统计学特征进行了统计学分析。
基于 18S rRNA 的 PCR 检测法的阳性率为 16%（200 例中有 32 例），均被归类为“基因型 1”。对 6 个 MLST 基因座的分析显示，只有一个基因座存在不同的单倍型，其余 5 个基因座没有多态性。本研究中的单倍型与至少一个先前报道的参考序列相同，除了“RNA 聚合酶 II 大亚基”基因座。在感染和未感染组之间，任何临床发现或人口统计学特征均无显著差异。
与包括意大利、丹麦、英国、澳大利亚和巴西在内的其他国家一样，本研究表明土耳其脆弱双核阿米巴分离株的遗传多样性较低。管家基因的高度序列相似性表明脆弱双核阿米巴呈克隆分布。
