González D, Zhao Q, McMahan C, Velasquez D, Haskins W E, Sponsel V, Cassill A, Renthal R
Department of Biology, University of Texas at San Antonio, USA.
Insect Mol Biol. 2009 Jun;18(3):395-404. doi: 10.1111/j.1365-2583.2009.00883.x.
Some chemosensory proteins (CSPs) are expressed in insect sensory appendages and are thought to be involved in chemical signalling by ants. We identified 14 unique CSP sequences in expressed sequence tag (EST) libraries of the red imported fire ant, Solenopsis invicta. One member of this group (Si-CSP1) is highly expressed in worker antennae, suggesting an olfactory function. A shotgun proteomic analysis of antennal proteins confirmed the high level of Si-CSP1 expression, and also showed expression of another CSP and two odorant-binding proteins (OBPs). We cloned and expressed the coding sequence for Si-CSP1. We used cyclodextrins as solubilizers to investigate ligand binding. Fire ant cuticular lipids strongly inhibited Si-CSP1 binding to the fluorescent dye N-phenyl-naphthylamine, suggesting cuticular substances are ligands for Si-CSP1. Analysis of the cuticular lipids showed that the endogenous ligands of Si-CSP1 are not cuticular hydrocarbons.
一些化学感受蛋白（CSPs）在昆虫的感觉附属器中表达，被认为参与蚂蚁的化学信号传导。我们在红火蚁（Solenopsis invicta）的表达序列标签（EST）文库中鉴定出14个独特的CSP序列。该组中的一个成员（Si-CSP1）在工蚁触角中高度表达，表明其具有嗅觉功能。对触角蛋白的鸟枪法蛋白质组分析证实了Si-CSP1的高表达水平，同时还显示了另一种CSP和两种气味结合蛋白（OBPs）的表达。我们克隆并表达了Si-CSP1的编码序列。我们使用环糊精作为增溶剂来研究配体结合。火蚁表皮脂质强烈抑制Si-CSP1与荧光染料N-苯基萘胺的结合，表明表皮物质是Si-CSP1的配体。对表皮脂质的分析表明，Si-CSP1的内源性配体不是表皮碳氢化合物。
