Peng Hanjing, Cheng Yunfeng, Ni Nanting, Li Minyong, Choudhary Gaurav, Chou Han Ting, Lu Chung-Dar, Tai Phang C, Wang Binghe
Department of Chemistry and Center for Biotechnology and Drug Design, Georgia State University, P.O. Box 4098, Atlanta, GA 30302-4098, USA.
ChemMedChem. 2009 Sep;4(9):1457-68. doi: 10.1002/cmdc.200900180.
Bacterial quorum sensing has received much attention in recent years because of its relevance to pathological events such as biofilm formation. Based on the structures of two lead inhibitors (IC50: 35-55 microM) against autoinducer-2-mediated quorum sensing identified through virtual screening, we synthesized 39 analogues and examined their inhibitory activities. Twelve of these new analogues showed equal or better inhibitory activities than the lead inhibitors. The best compound showed an IC50 value of approximately 6 microM in a whole-cell assay using Vibrio harveyi as the model organism. The structure-activity relationship is discussed herein.
近年来，细菌群体感应因其与生物膜形成等病理事件相关而备受关注。基于通过虚拟筛选确定的两种针对自诱导物-2介导的群体感应的先导抑制剂（IC50：35 - 55 microM）的结构，我们合成了39种类似物并检测了它们的抑制活性。这些新类似物中有12种表现出与先导抑制剂相当或更好的抑制活性。在以哈维弧菌为模式生物的全细胞测定中，最佳化合物的IC50值约为6 microM。本文讨论了构效关系。
