基于阳离子共轭聚合物与分支DNA组装的荧光逻辑信号核酸酶多重检测
Fluorescence logic-signal-based multiplex detection of nucleases with the assembly of a cationic conjugated polymer and branched DNA.
作者信息
Feng Xuli, Duan Xinrui, Liu Libing, Feng Fude, Wang Shu, Li Yuliang, Zhu Daoben
机构信息
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190 PR China.
出版信息
Angew Chem Int Ed Engl. 2009;48(29):5316-21. doi: 10.1002/anie.200901555.
An energy-transfer cascade is generated from a cationic conjugated polymer (PFP) and negatively charged, Y-shaped DNA labeled with three dyes at its termini (fluorescein (Fl), Tex Red, and Cy5). Multistep fluorescence resonance energy transfer regulates the fluorescence intensities of PFP and the dyes. Different types of logic gates can be operated by observing the emission wavelengths of different dyes with multiplex nucleases as inputs.
由阳离子共轭聚合物（PFP）和末端标记有三种染料（荧光素（Fl）、德克萨斯红和Cy5）的带负电荷的Y形DNA产生能量转移级联。多步荧光共振能量转移调节PFP和染料的荧光强度。通过以多重核酸酶作为输入观察不同染料的发射波长，可以操作不同类型的逻辑门。
Zotero 插件