使用傅里叶域锁模激光器的扫频源光学相干显微镜。

Swept source optical coherence microscopy using a Fourier domain mode-locked laser.

作者信息

Huang Shu-Wei, Aguirre Aaron D, Huber Robert A, Adler Desmond C, Fujimoto James G

机构信息

Department of Electrical Engineering and Computer Science and Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

出版信息

Opt Express. 2007 May 14;15(10):6210-7. doi: 10.1364/oe.15.006210.

DOI:10.1364/oe.15.006210

PMID:19546926

Abstract

Swept source optical coherence microscopy (OCM) enables cellular resolution en face imaging as well as integration with optical coherence tomography (OCT) cross sectional imaging. A buffered Fourier domain mode-locked (FDML) laser light source provides high speed, three dimensional imaging. Image resolutions of 1.6 microm x 8 microm (transverse x axial) with a 220 microm x 220 microm field of view and sensitivity higher than 98 dB are achieved. Three dimensional cellular imaging is demonstrated in vivo in the Xenopus laevis tadpole and ex vivo in the rat kidney and human colon.

摘要

扫频源光学相干显微镜（OCM）能够实现细胞分辨率的正面成像以及与光学相干断层扫描（OCT）横截面成像的整合。一种缓冲傅里叶域锁模（FDML）激光光源可提供高速三维成像。在220微米×220微米的视野下实现了1.6微米×8微米（横向×轴向）的图像分辨率，灵敏度高于98分贝。在非洲爪蟾蝌蚪体内以及大鼠肾脏和人类结肠体外均展示了三维细胞成像。