Rezvanfar Mohammad Amin, Farshid Amir Abbas, Sadrkhanlou Rajab Ali, Ahmadi Abbas, Rezvanfar Mohammad Ali, Salehnia Alinazar, Abdollahi Mohammad
Laboratory of Histology, Department of Embryology and Histology, Faculty of Veterinary Medicine, Urmia University, Iran.
Exp Toxicol Pathol. 2010 May;62(3):323-30. doi: 10.1016/j.etp.2009.05.005. Epub 2009 Jun 23.
Cyclophosphamide (CP) as a widely used antineoplastic drug causes hemorrhagic cystitis (HC) mainly via induction of oxidative stress. Regarding established antioxidant potential of Satureja khuzestanica (Lamiaceae) essential oil (SKEO), we aimed to investigate its protective effects in a subchronic rat model of CP-induced HC. CP (6mg/kg/day) and SKEO (225mg/kg/day) were administered alone or in combination by gavage for 28 days. Histopathological changes were investigated by light microscopy. Plasma samples were assayed for lipid peroxidation and total antioxidant power as biomarkers of toxic stress. In the CP-treated animals, irregular mucus layer, severe hemorrhage and edema, infiltration of inflammatory cells, and accumulation of mast cells were observed. In the CP+SKEO group, a relatively normal urothelial topography with decreased number of mucosal mast cells and inflammatory cells were observed. Increased lipid peroxidation along with decreased total antioxidant capacity resulting from CP treatment was significantly recovered by SKEO co-treatment. It is concluded that SKEO protects rats from CP-induced HC by reduction of free radical-induced toxic stress. It is strongly recommended to examine SKEO in the clinic to approve its benefit in patients undertaking CP.
环磷酰胺（CP）作为一种广泛使用的抗肿瘤药物，主要通过诱导氧化应激导致出血性膀胱炎（HC）。鉴于胡齐斯坦鼠尾草（唇形科）精油（SKEO）已确定的抗氧化潜力，我们旨在研究其在CP诱导的HC亚慢性大鼠模型中的保护作用。CP（6mg/kg/天）和SKEO（225mg/kg/天）单独或联合灌胃给药28天。通过光学显微镜研究组织病理学变化。检测血浆样本中的脂质过氧化和总抗氧化能力，作为毒性应激的生物标志物。在CP处理的动物中，观察到黏液层不规则、严重出血和水肿、炎症细胞浸润以及肥大细胞积聚。在CP+SKEO组中，观察到尿路上皮形态相对正常，黏膜肥大细胞和炎症细胞数量减少。CP处理导致的脂质过氧化增加以及总抗氧化能力降低，通过SKEO联合处理得到显著恢复。结论是，SKEO通过减少自由基诱导的毒性应激保护大鼠免受CP诱导的HC。强烈建议在临床上检验SKEO，以证实其对接受CP治疗患者的益处。
