Postgraduate Program in Health Science and Development in the Central Western Region, Universidade Federal do Mato Grosso do Sul (UFMS), Carandá Bosque, 79032-423 Campo Grande, MS, Brazil.
Lasers Med Sci. 2010 Jan;25(1):115-20. doi: 10.1007/s10103-009-0697-9. Epub 2009 Jun 25.
Studies suggest that high-intensity physical exercise can cause damage to skeletal muscles, resulting in muscle soreness, fatigue, inflammatory processes and cell apoptosis. The aim of this study was to investigate the effects of low-level laser therapy (LLLT) on a decrease in creatine kinase (CK) levels and cell apoptosis. Twenty male Wistar rats were randomly divided into two equal groups: group 1 (control), resistance swimming; group 2 (LLLT), resistance swimming with LLLT. They were subjected to a single application of indium gallium aluminum phosphide (InGaAlP) laser immediately following the exercise for 40 s at an output power of 100 mW, wavelength 660 nm and 133.3 J/cm(2). The groups were subdivided according to sample collection time: 24 h and 48 h. CK was measured before and both 24 h and 48 h after the test. Samples of the gastrocnemius muscle were processed to determine the presence of apoptosis using terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP) nick end labeling. (There was a significant difference in CK levels between groups (P < 0.0001) as well as between the 24 h and 48 h levels in the control group, whereas there was no significant intra-group difference in the LLLT group at the same evaluation times. In the LLLT group there were 66.3 +/- 13.2 apoptotic cells after 24 h and 39.0 +/- 6.8 apoptotic cells after 48 h. The results suggest that LLLT influences the metabolic profile of animals subjected to fatigue by lowering serum levels of CK. This demonstrates that LLLT can act as a preventive tool against cell apoptosis experienced during high-intensity physical exercise.
研究表明，高强度的体育锻炼会对骨骼肌造成损伤，导致肌肉酸痛、疲劳、炎症过程和细胞凋亡。本研究旨在探讨低水平激光疗法（LLLT）对降低肌酸激酶（CK）水平和细胞凋亡的影响。20 只雄性 Wistar 大鼠随机分为两组：对照组（阻力游泳）和 LLLT 组（阻力游泳加 LLLT）。运动后立即对它们进行单次铟镓铝磷（InGaAlP）激光照射，输出功率为 100 mW，波长 660nm，能量密度为 133.3J/cm2，照射时间为 40s。两组根据采样时间进一步分为 24h 和 48h 两个亚组。在试验前后分别测量 CK，在 24h 和 48h 后测量 CK。使用末端脱氧核苷酸转移酶（TdT）介导的脱氧尿苷三磷酸（dUTP）缺口末端标记法处理腓肠肌样本，以确定是否存在细胞凋亡。（组间 CK 水平存在显著差异（P<0.0001），对照组 24h 和 48h 水平也存在显著差异，而 LLLT 组在同一评估时间内组内差异无统计学意义。LLLT 组在 24h 后有 66.3±13.2 个凋亡细胞，在 48h 后有 39.0±6.8 个凋亡细胞。结果表明，LLLT 通过降低血清 CK 水平影响疲劳动物的代谢谱。这表明 LLLT 可作为高强度体育锻炼中预防细胞凋亡的工具。
