Department of Neuroscience and Ophthalmology, Institute of Inflammation and Ageing, University of Birmingham, Birmingham, United Kingdom.
National Institute of Health Research Surgical Reconstruction and Microbiology Research Centre, University Hospitals Birmingham' Edgbaston, Birmingham, United Kingdom.
PLoS One. 2022 Mar 3;17(3):e0264533. doi: 10.1371/journal.pone.0264533. eCollection 2022.
Apoptotic cell death within the brain represents a significant contributing factor to impaired post-traumatic tissue function and poor clinical outcome after traumatic brain injury. After irradiation with light in the wavelength range of 600-1200 nm (photobiomodulation), previous investigations have reported a reduction in apoptosis in various tissues. This study investigates the effect of 660 nm photobiomodulation on organotypic slice cultured hippocampal tissue of rats, examining the effect on apoptotic cell loss. Tissue optical Raman spectroscopic changes were evaluated. A significantly higher proportion of apoptotic cells 62.8±12.2% vs 48.6±13.7% (P<0.0001) per region were observed in the control group compared with the photobiomodulation group. After photobiomodulation, Raman spectroscopic observations demonstrated 1440/1660 cm-1 spectral shift. Photobiomodulation has the potential for therapeutic utility, reducing cell loss to apoptosis in injured neurological tissue, as demonstrated in this in vitro model. A clear Raman spectroscopic signal was observed after apparent optimal irradiation, potentially integrable into therapeutic light delivery apparatus for real-time dose metering.
脑内细胞凋亡是创伤性脑损伤后组织功能受损和临床预后不良的一个重要因素。在波长范围为 600-1200nm 的光照射下（光生物调节），先前的研究报告称，在各种组织中细胞凋亡减少。本研究调查了 660nm 光生物调节对大鼠器官型脑片培养海马组织的影响，研究了对细胞凋亡丢失的影响。评估了组织光学拉曼光谱的变化。与光生物调节组相比，对照组每个区域的凋亡细胞比例明显更高，分别为 62.8±12.2%和 48.6±13.7%（P<0.0001）。光生物调节后，拉曼光谱观察到 1440/1660cm-1 光谱位移。光生物调节具有治疗用途的潜力，可减少神经损伤组织中的细胞凋亡损失，本体外模型证实了这一点。在明显的最佳照射后观察到清晰的拉曼光谱信号，可能集成到治疗性光输送设备中，用于实时剂量测量。
