Vasconcelos Alan B, Nampo Fernando K, Molina Júlio C, Silva Miriam B, Oliveira Alan S, de Angelis Tarlyson R, Hasuda Amanda L, Camargo Enilton A, Ramos Solange P
Department of Physiology, Federal University of Sergipe, Marechal Rondon Av., São Cristóvão, SE, CEP 49100-000, Brazil.
Latin American Institute of Life and Natural Sciences, Federal University of Latin American Integration, Foz do Iguaçu, PR, Brazil.
Lasers Med Sci. 2019 Jun;34(4):749-758. doi: 10.1007/s10103-018-2655-x. Epub 2018 Oct 17.
We compared the acute effects of different doses of 630 nm light-emitting diode therapy (LEDT) on skeletal muscle inflammation and hyperalgesia in rats submitted to exercise-induced muscle damage (EIMD). Wistar rats were divided into five experimental groups (n = 5-8/group): sedentary control (CON); exercise + passive recovery (PR); and exercise + LEDT (1.2 J/cm, 1.8 J; 4.2 J/cm, 6.3 J; 10.0 J/cm, 15 J). After 100 min of swimming, the rats in the LEDT groups were exposed to phototherapy on the triceps surae muscle. For mechanical hyperalgesia evaluation, paw withdrawal threshold was assessed before and 24 h after swimming. Immediately after hyperalgesia tests, blood samples were collected to analyze creatine kinase (CK) activity and the soleus muscle was removed for histological and tumor necrosis factor (TNF)-α immunohistological analyses. In all LEDT groups, plasma CK activity was reduced to levels similar to those measured in the CON group. Paw withdrawal threshold decreased in the PR group (- 11.9 ± 1.9 g) when compared to the CON group (2.2 ± 1.5 g; p < 0.01) and it was attenuated in the group LEDT 4.2 J/cm (- 3.3 ± 2.4 g, p < 0.05). Less leukocyte infiltration and edema and fewer necrotic areas were found in histological sections of soleus muscle in LEDT (4.2 J/cm) and LEDT (10.0 J/cm) groups compared to the PR group. Also, LEDT (4.2 J/cm) and LEDT (10.0 J/cm) groups showed less immunostaining for TNF-α in macrophages or areas with necrosis of muscle fibers compared to the PR group. LEDT (4.2 J/cm, 6.3 J)-reduced muscle inflammation and nociception in animals submitted to EIMD.
我们比较了不同剂量的630纳米发光二极管疗法（LEDT）对运动诱导的肌肉损伤（EIMD）大鼠骨骼肌炎症和痛觉过敏的急性影响。将Wistar大鼠分为五个实验组（每组n = 5 - 8）：久坐对照组（CON）；运动 + 被动恢复组（PR）；以及运动 + LEDT组（1.2 J/cm²，1.8 J；4.2 J/cm²，6.3 J；10.0 J/cm²，15 J）。游泳100分钟后，LEDT组的大鼠在腓肠肌接受光疗。为了评估机械性痛觉过敏，在游泳前和游泳后24小时评估爪部撤离阈值。在痛觉过敏测试后立即采集血样以分析肌酸激酶（CK）活性，并取出比目鱼肌进行组织学和肿瘤坏死因子（TNF）-α免疫组织学分析。在所有LEDT组中，血浆CK活性降低至与CON组测量水平相似。与CON组（2.2±1.5 g）相比，PR组的爪部撤离阈值降低（-11.9±1.9 g；p < 0.01），而在LEDT 4.2 J/cm²组中该阈值有所减轻（-3.3±2.4 g，p < 0.05）。与PR组相比，LEDT（4.2 J/cm²）组和LEDT（10.0 J/cm²）组的比目鱼肌组织切片中白细胞浸润和水肿较少，坏死区域也较少。此外，与PR组相比，LEDT（4.2 J/cm²）组和LEDT（10.0 J/cm²）组在巨噬细胞或肌纤维坏死区域的TNF-α免疫染色较少。LEDT（4.2 J/cm²，6.3 J）可减轻EIMD动物的肌肉炎症和伤害感受。
