Kotze Andrew C, Lowe Ann, O'Grady John, Kopp Steven R, Behnke Jerzy M
CSIRO Livestock Industries, St. Lucia, Brisbane, Australia.
Am J Trop Med Hyg. 2009 Jul;81(1):163-70.
With the implementation of mass drug administration programs for the control of human hookworms, there is a need to monitor for the emergence of drug resistance. We have therefore examined in vitro assays for monitoring sensitivity to benzimidazoles (egg hatch assay) and nicotinic acetylcholine receptor agonist drugs (motility and morphology assays), with a view to developing tools for monitoring drug sensitivity in the field. We have performed assays with Necator americanus and Ancylostoma ceylanicum, and combined this with published data on N. americanus and Ancylostoma caninum, to indicate the breadth of the responses of various hookworm species and isolates in these in vitro assays. This has allowed us to generate assay templates covering the known range of responses, with scope to cover any shift in response that may be indicative of resistance. These assays will have immediate applicability in monitoring for the emergence of drug resistance in human hookworm populations.
随着用于控制人体钩虫的群体药物给药计划的实施，有必要监测耐药性的出现。因此，我们研究了用于监测对苯并咪唑类药物（虫卵孵化试验）和烟碱型乙酰胆碱受体激动剂药物（运动和形态学试验）敏感性的体外试验，以期开发出用于在现场监测药物敏感性的工具。我们用美洲板口线虫和锡兰钩口线虫进行了试验，并将其与已发表的关于美洲板口线虫和犬钩口线虫的数据相结合，以表明在这些体外试验中各种钩虫种类和分离株的反应范围。这使我们能够生成涵盖已知反应范围的试验模板，并有能力涵盖任何可能表明耐药性的反应变化。这些试验将在监测人体钩虫群体中耐药性的出现方面立即得到应用。
