Douglas R M, Roberts J A, Munro A W, Ritchie G Y, Lamb A J, Booth I R
Department of Molecular and Cell Biology, University of Aberdeen, Marischal College, UK.
J Gen Microbiol. 1991 Aug;137(8):1999-2005. doi: 10.1099/00221287-137-8-1999.
Using a variety of techniques the distribution of the glutathione-regulated KefC K(+)-transport system among bacterial species was investigated. The presence of similar systems in a number of Gram-negative bacteria was demonstrated. In contrast, the system appeared to be absent from most Gram-positive bacteria tested with the exception of Staphylococcus aureus. Using the cloned Escherichia coli kefC gene as a probe for Southern hybridization it was shown that only limited DNA sequence homology exists with other bacteria, even when closely related members of the enteric group were examined.
运用多种技术研究了谷胱甘肽调节的KefC钾离子转运系统在细菌物种间的分布情况。结果表明，许多革兰氏阴性菌中存在类似系统。相比之下，除金黄色葡萄球菌外，大多数受试革兰氏阳性菌中似乎不存在该系统。以克隆的大肠杆菌kefC基因作为Southern杂交探针，结果显示，即便检测肠道菌群中亲缘关系较近的成员，与其他细菌的DNA序列同源性也很有限。
