鉴定大肠杆菌中KefC谷胱甘肽门控钾离子外流系统活性所需的辅助蛋白YabF。
Identification of an ancillary protein, YabF, required for activity of the KefC glutathione-gated potassium efflux system in Escherichia coli.
作者信息
Miller S, Ness L S, Wood C M, Fox B C, Booth I R
机构信息
Department of Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, United Kingdom.
出版信息
J Bacteriol. 2000 Nov;182(22):6536-40. doi: 10.1128/JB.182.22.6536-6540.2000.
Abstract
A new subunit, YabF, for the KefC K(+) efflux system in Escherichia coli has been identified. The subunit is required for maximum activity of KefC. Deletion of yabF reduces KefC activity 10-fold, and supply of YabF in trans restores activity. IS2 and IS10R insertions in yabF can be isolated as suppressors of KefC activity consequent upon the V427A and D264A KefC mutations.
摘要
已鉴定出大肠杆菌中KefC钾离子外流系统的一个新亚基YabF。该亚基是KefC发挥最大活性所必需的。缺失yabF会使KefC活性降低10倍，而反式提供YabF可恢复活性。yabF中的IS2和IS10R插入可作为V427A和D264A KefC突变导致的KefC活性抑制子被分离出来。
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