鉴定大肠杆菌中KefC谷胱甘肽门控钾离子外流系统活性所需的辅助蛋白YabF。
Identification of an ancillary protein, YabF, required for activity of the KefC glutathione-gated potassium efflux system in Escherichia coli.
作者信息
Miller S, Ness L S, Wood C M, Fox B C, Booth I R
机构信息
Department of Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, United Kingdom.
出版信息
J Bacteriol. 2000 Nov;182(22):6536-40. doi: 10.1128/JB.182.22.6536-6540.2000.
Abstract
A new subunit, YabF, for the KefC K(+) efflux system in Escherichia coli has been identified. The subunit is required for maximum activity of KefC. Deletion of yabF reduces KefC activity 10-fold, and supply of YabF in trans restores activity. IS2 and IS10R insertions in yabF can be isolated as suppressors of KefC activity consequent upon the V427A and D264A KefC mutations.
摘要
已鉴定出大肠杆菌中KefC钾离子外流系统的一个新亚基YabF。该亚基是KefC发挥最大活性所必需的。缺失yabF会使KefC活性降低10倍，而反式提供YabF可恢复活性。yabF中的IS2和IS10R插入可作为V427A和D264A KefC突变导致的KefC活性抑制子被分离出来。
相似文献
1
Identification of an ancillary protein, YabF, required for activity of the KefC glutathione-gated potassium efflux system in Escherichia coli.
J Bacteriol. 2000 Nov;182(22):6536-40. doi: 10.1128/JB.182.22.6536-6540.2000.
2
Mutations in the glutathione-gated KefC K+ efflux system of Escherichia coli that cause constitutive activation.
J Biol Chem. 1997 Oct 3;272(40):24942-7. doi: 10.1074/jbc.272.40.24942.
3
The K(+)-efflux system, KefC, in Escherichia coli: genetic evidence for oligomeric structure.
Mol Membr Biol. 1994 Jan-Mar;11(1):55-61. doi: 10.3109/09687689409161030.
4
Different foci for the regulation of the activity of the KefB and KefC glutathione-gated K+ efflux systems.
J Biol Chem. 1999 Apr 2;274(14):9524-30. doi: 10.1074/jbc.274.14.9524.
5
Survival of Escherichia coli cells exposed to iodoacetate and chlorodinitrobenzene is independent of the glutathione-gated K+ efflux systems KefB and KefC.
Appl Environ Microbiol. 1997 Oct;63(10):4083-6. doi: 10.1128/aem.63.10.4083-4086.1997.
6
The cloning and DNA sequence of the gene for the glutathione-regulated potassium-efflux system KefC of Escherichia coli.
Mol Microbiol. 1991 Mar;5(3):607-16. doi: 10.1111/j.1365-2958.1991.tb00731.x.
7
The distribution of homologues of the Escherichia coli KefC K(+)-efflux system in other bacterial species.
J Gen Microbiol. 1991 Aug;137(8):1999-2005. doi: 10.1099/00221287-137-8-1999.
8
The role of glyoxalase I in the detoxification of methylglyoxal and in the activation of the KefB K+ efflux system in Escherichia coli.
Mol Microbiol. 1998 Feb;27(3):563-71. doi: 10.1046/j.1365-2958.1998.00701.x.
9
Survival during exposure to the electrophilic reagent N-ethylmaleimide in Escherichia coli: role of KefB and KefC potassium channels.
J Bacteriol. 1997 Feb;179(4):1007-12. doi: 10.1128/jb.179.4.1007-1012.1997.
10
Glutathione-gated K+ channels of Escherichia coli carry out K+ efflux controlled by the redox state of the cell.
Arch Microbiol. 1990;154(5):475-82. doi: 10.1007/BF00245231.
引用本文的文献
1
Interaction of unphosphorylated PtsN with the K/H antiporter YcgO inhibits its activity in Escherichia coli.
J Biol Chem. 2025 Feb;301(2):108153. doi: 10.1016/j.jbc.2024.108153. Epub 2024 Dec 30.
2
The Potassium Efflux System Kef: Bacterial Protection against Toxic Electrophilic Compounds.
Membranes (Basel). 2023 Apr 27;13(5):465. doi: 10.3390/membranes13050465.
3
Silencing of and Revealed Their Potential Functions Under Salt and Potassium Stresses in Upland Cotton.
Front Plant Sci. 2021 Dec 7;12:789775. doi: 10.3389/fpls.2021.789775. eCollection 2021.
4
A Holling Functional Response Model for Mapping QTLs Governing Interspecific Interactions.
Front Genet. 2021 Oct 15;12:766372. doi: 10.3389/fgene.2021.766372. eCollection 2021.
5
Diverse Physiological Functions of Cation Proton Antiporters across Bacteria and Plant Cells.
Int J Mol Sci. 2020 Jun 26;21(12):4566. doi: 10.3390/ijms21124566.
6
Genetic and molecular characterization of multicomponent resistance of against allicin.
Life Sci Alliance. 2020 Mar 31;3(5). doi: 10.26508/lsa.202000670. Print 2020 May.
7
Untargeted metabolomics links glutathione to bacterial cell cycle progression.
Nat Metab. 2020 Feb;2(2):153-166. doi: 10.1038/s42255-019-0166-0. Epub 2020 Feb 3.
8
Evidence for potassium transport activity of Arabidopsis KEA1-KEA6.
Sci Rep. 2019 Jul 11;9(1):10040. doi: 10.1038/s41598-019-46463-7.
9
Kup-mediated Cs uptake and Kdp-driven K uptake coordinate to promote cell growth during excess Cs conditions in Escherichia coli.
Sci Rep. 2017 May 18;7(1):2122. doi: 10.1038/s41598-017-02164-7.
10
Evidence for plasmid-mediated salt tolerance in the human gut microbiome and potential mechanisms.
FEMS Microbiol Ecol. 2016 Mar;92(3). doi: 10.1093/femsec/fiw019. Epub 2016 Feb 4.
本文引用的文献
1
Crystal structure of human quinone reductase type 2, a metalloflavoprotein.
Biochemistry. 1999 Aug 3;38(31):9881-6. doi: 10.1021/bi990799v.
2
Different foci for the regulation of the activity of the KefB and KefC glutathione-gated K+ efflux systems.
J Biol Chem. 1999 Apr 2;274(14):9524-30. doi: 10.1074/jbc.274.14.9524.
3
Linkage map of Escherichia coli K-12, edition 10: the physical map.
Microbiol Mol Biol Rev. 1998 Sep;62(3):985-1019. doi: 10.1128/MMBR.62.3.985-1019.1998.
4
Linkage map of Escherichia coli K-12, edition 10: the traditional map.
Microbiol Mol Biol Rev. 1998 Sep;62(3):814-984. doi: 10.1128/MMBR.62.3.814-984.1998.
5
From famine to feast: the role of methylglyoxal production in Escherichia coli.
Mol Microbiol. 1998 Feb;27(3):553-62. doi: 10.1046/j.1365-2958.1998.00700.x.
6
Mutations in the glutathione-gated KefC K+ efflux system of Escherichia coli that cause constitutive activation.
J Biol Chem. 1997 Oct 3;272(40):24942-7. doi: 10.1074/jbc.272.40.24942.
7
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
Nucleic Acids Res. 1997 Sep 1;25(17):3389-402. doi: 10.1093/nar/25.17.3389.
8
The K(+)-efflux system, KefC, in Escherichia coli: genetic evidence for oligomeric structure.
Mol Membr Biol. 1994 Jan-Mar;11(1):55-61. doi: 10.3109/09687689409161030.
9
Shaker K+ channel beta subunits belong to an NAD(P)H-dependent oxidoreductase superfamily.
Cell. 1994 Dec 30;79(7):1133-5. doi: 10.1016/0092-8674(94)90004-3.
10
Activation of potassium channels during metabolite detoxification in Escherichia coli.
Mol Microbiol. 1993 Sep;9(6):1297-303. doi: 10.1111/j.1365-2958.1993.tb01259.x.
Zotero 插件