Gazda Agnieszka, Zabek Jakub, Romicka Anna M, Wojciechowska Bozena, Pyka Joanna
Department of Pediatrician Rheumatology, Eleonora Reicher Rheumatology Institute, Warszawa, Poland.
Pol Arch Med Wewn. 2008;118 Suppl:14-9.
The presence of antiphospholipid antibodies (APA), especially anticardiolipin antibodies (ACA), antibodies against beta2-glikoprotein I and lupus anticoagulant leads to thrombotic disorders. The pathogenetic role of APA in children is not exactly explained. The frequency of occurrence of APA and antiphospholipid syndrom in children is 2 to 3 times lower than in adults.
The aim of the study was to indicate the presence of ACA in circulating immunological complexes (CIC) from sera of patients suffering from juvenile idiopathic arthritis (JIA) and juvenile systemic lupus erythematosus (JSLE).
31 sera were investigated, 16 from JIA patients and 15 from JSLE patients. The free ACA IgG class, ACA bound in CIC were estimated by the ELISA method (after dissociation of CIC and y-fraction paecipitation).
In CIC isolated from sera of JIA and JSLE patients ACA were discovered. ACA, possessing high avidity, in CIC were more frequently discovered than unbound ACA (38.7% vs. 25.8%).
ACA in CIC were more frequent in sera of JIA patients, unbound ACA were more frequent in sera of JSLE patients.
抗磷脂抗体（APA）的存在，尤其是抗心磷脂抗体（ACA）、抗β2糖蛋白I抗体和狼疮抗凝物会导致血栓形成性疾病。APA在儿童中的致病作用尚未完全阐明。儿童中APA和抗磷脂综合征的发生率比成人低2至3倍。
本研究旨在指出幼年特发性关节炎（JIA）和幼年系统性红斑狼疮（JSLE）患者血清循环免疫复合物（CIC）中ACA的存在情况。
研究了31份血清，其中16份来自JIA患者，15份来自JSLE患者。通过ELISA法（CIC解离和γ-组分沉淀后）估计游离ACA IgG类、CIC中结合的ACA。
在从JIA和JSLE患者血清中分离出的CIC中发现了ACA。CIC中具有高亲和力的ACA比未结合的ACA更常被发现（38.7%对25.8%）。
JIA患者血清中CIC中的ACA更常见，JSLE患者血清中未结合的ACA更常见。
