Hasturk Hatice, Goguet-Surmenian Emilie, Blackwood Amanda, Andry Chris, Kantarci Alpdogan
Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, Boston, MA, USA.
J Periodontol. 2009 Jul;80(7):1103-13. doi: 10.1902/jop.2009.090002.
The present study was planned to investigate the therapeutic actions of 1-tetradecanol complex (1-TDC), a novel monounsaturated fatty acid mixture, in established periodontitis in rabbits.
Periodontitis was initiated in 18 New Zealand White rabbits using ligatures around mandibular second premolars, followed by topical Porphyromonas gingivalis application (10(9) colony forming units). After 6 weeks of disease induction (phase 1), three animals were sacrificed to assess the established periodontitis level. P. gingivalis application was discontinued, and the remaining 15 animals continued with topical treatment of 1-TDC (100 mg/ml; n = 5) or placebo (n = 5) or no treatment (n = 5) for an additional 6 weeks (phase 2). Mandibular block sections obtained after euthanasia were decalcified and embedded in paraffin. In addition to the macroscopic analyses, hematoxylin and eosin-stained sections were used to study cellular inflammatory infiltrate and quantitative histomorphometry. Tartrate-resistant acid phosphatase and osteocalcin were used to identify osteoclastic and osteoblastic activity, respectively.
P. gingivalis application resulted in periodontal disease with gingival inflammation and bone loss (30% compared to baseline) at 6 weeks. Treatment with 1-TDC stopped the progression of the disease and resulted in a significant reduction in the macroscopic periodontal inflammation, attachment, and bone loss (10.1% +/- 1.8%), whereas periodontal disease progressed in the untreated and placebo groups (P <0.05). Histologic assessment and histomorphometric measurements demonstrated that 1-TDC inhibited inflammatory cell infiltration and osteoclastic activity (P <0.05).
The findings suggest that topical application of cetylated monounsaturated fatty acid complex (1-TDC) is a potential therapeutic approach in controlling the progression of chronic periodontal disease.
本研究旨在探讨新型单不饱和脂肪酸混合物1-十四醇复合物（1-TDC）对兔实验性牙周炎的治疗作用。
对18只新西兰白兔的下颌第二前磨牙使用结扎丝诱导牙周炎，随后局部应用牙龈卟啉单胞菌（10⁹ 菌落形成单位）。疾病诱导6周后（第1阶段），处死3只动物以评估已形成的牙周炎水平。停止应用牙龈卟啉单胞菌，其余15只动物继续局部应用1-TDC（100 mg/ml；n = 5）或安慰剂（n = 5）或不治疗（n = 5），持续6周（第2阶段）。安乐死后获取的下颌骨组织块经脱钙后包埋于石蜡中。除宏观分析外，苏木精-伊红染色切片用于研究细胞炎性浸润和定量组织形态计量学。分别使用抗酒石酸酸性磷酸酶和骨钙素鉴定破骨细胞和成骨细胞活性。
应用牙龈卟啉单胞菌6周时导致牙周疾病，伴有牙龈炎症和骨吸收（与基线相比减少30%）。1-TDC治疗阻止了疾病进展，使宏观牙周炎症、附着丧失和骨吸收显著减少（10.1%±1.8%），而未治疗组和安慰剂组的牙周疾病继续进展（P<0.05）。组织学评估和组织形态计量学测量表明，1-TDC抑制炎性细胞浸润和破骨细胞活性（P<0.05）。
研究结果表明，局部应用十六烷基化单不饱和脂肪酸复合物（1-TDC）是控制慢性牙周疾病进展的一种潜在治疗方法。
