Khdera Hadi Aqel, Saad Sawsan Youseff
Department of Chemistry, Faculty of Sciences, Tishreen University, Lattakia, Syria.
Heliyon. 2024 Jul 16;10(14):e34686. doi: 10.1016/j.heliyon.2024.e34686. eCollection 2024 Jul 30.
L. is a perennial herbaceous plant belonging to the Verbenaceae family. It is widely used as an herbal drink to treat many diseases. It has antioxidant, antifungal and anti-inflammatory properties. In traditional medicine, it is used to treat skin infections. However, there is little information on the chemical composition of organic plant extracts. Therefore, the aim of this study was to determine the chemical composition of organic extracts of L.
In this study, organic extracts were prepared using a continuous Soxhlet extractor and four different solvents with increasing polarity from nonpolar to polar solvents (petroleum ether, chloroform, ethyl acetate, and isopropanol) to ensure the possibility of extracting a wide range of compounds. GC‒MS analysis was performed to determine the chemical constituents of the organic extracts.
Nineteen compounds were identified in the petroleum ether (Et) extract, 14 in the chloroform (Ch) extract, 18 in the ethyl acetate (Ea) extract and 15 in the isopropanol (Is) extract. The most important compounds in the Et extract were 1,1-diethoxyethane (33.9 %) and nonadecane (19.9 %). The most important compound in the Ch extract was octacosane (37.4 %). The most important compounds in the Ea extract were 3-hydroxy-dodecanoic acid (17.7 %) and geranyl isovalerate (15.5 %). The most important compound in the Is extract was behenic acid alcohol (18.6 %). The chemical structures of the major compounds were confirmed by mass spectrometry by studying their fragmentation mechanism and comparing the molecular weights of the resulting fragments with the molecular weights of the peaks present in each mass spectrum.
The results of this study show that the dominant compounds in nonpolar extracts (petroleum ether and chloroform) are hydrocarbons, ethers, epoxides, and silicon compounds, while the dominant compounds in moderately polar extracts (ethyl acetate and isopropanol) are alcohols, carbonyl compounds, and oxygenated terpenes.
L. 是一种属于马鞭草科的多年生草本植物。它被广泛用作草药饮品来治疗多种疾病。它具有抗氧化、抗真菌和抗炎特性。在传统医学中，它被用于治疗皮肤感染。然而，关于有机植物提取物的化学成分的信息很少。因此，本研究的目的是确定L. 的有机提取物的化学成分。
在本研究中，使用连续索氏提取器和四种极性从非极性到极性递增的不同溶剂（石油醚、氯仿、乙酸乙酯和异丙醇）制备有机提取物，以确保提取广泛化合物的可能性。进行气相色谱 - 质谱联用（GC - MS）分析以确定有机提取物的化学成分。
在石油醚（Et）提取物中鉴定出19种化合物，氯仿（Ch）提取物中鉴定出14种，乙酸乙酯（Ea）提取物中鉴定出18种，异丙醇（Is）提取物中鉴定出15种。Et提取物中最重要的化合物是1,1 - 二乙氧基乙烷（33.9%）和十九烷（19.9%）。Ch提取物中最重要的化合物是二十八烷（37.4%）。Ea提取物中最重要的化合物是3 - 羟基 - 十二烷酸（17.7%）和香叶基异戊酸酯（15.5%）。Is提取物中最重要的化合物是山嵛醇（18.6%）。通过研究其裂解机制并将所得碎片的分子量与每个质谱图中存在的峰的分子量进行比较，通过质谱法确认了主要化合物的化学结构。
本研究结果表明，非极性提取物（石油醚和氯仿）中的主要化合物是烃类、醚类、环氧化物和硅化合物，而中等极性提取物（乙酸乙酯和异丙醇）中的主要化合物是醇类、羰基化合物和氧化萜类。
