Wu Liangxing, Loudet Aurore, Barhoumi Rola, Burghardt Robert C, Burgess Kevin
Department of Chemistry, Texas A & M University, Box 30012, College Station, Texas 77841, USA.
J Am Chem Soc. 2009 Jul 8;131(26):9156-7. doi: 10.1021/ja9029413.
Two water-soluble "through-bond energy transfer cassettes" (TBET-cassettes) were prepared. They have good extinction coefficients at the donor part and transfer energy to the acceptor parts with good "overall quantum yields" (0.30 and 0.24 in pH 7.4 phosphate buffer). Fluorescence resonance energy transfer from one protein functionalized with an appropriate donor can be used to excite the TBET-cassettes on different proteins to probe protein-protein interactions under conditions that would not be possible for single-dye acceptor systems.
制备了两种水溶性的“通过化学键的能量转移盒”（TBET盒）。它们在供体部分具有良好的消光系数，并以良好的“总量子产率”（在pH 7.4磷酸盐缓冲液中分别为0.30和0.24）将能量转移到受体部分。来自用合适供体功能化的一种蛋白质的荧光共振能量转移可用于激发不同蛋白质上的TBET盒，以在单染料受体系统无法实现的条件下探测蛋白质-蛋白质相互作用。
