Department of Biochemistry and Nutrition, Central Food Technological Research Institute, CSIR, Mysore 570 020, Karnataka, India.
Evid Based Complement Alternat Med. 2011;2011:249487. doi: 10.1093/ecam/nep060. Epub 2011 Jun 23.
Zinger officinale has been used as a traditional source against gastric disturbances from time immemorial. The ulcer-preventive properties of aqueous extract of ginger rhizome (GRAE) belonging to the family Zingiberaceae is reported in the present study. GRAE at 200 mg kg(-1) b.w. protected up to 86% and 77% for the swim stress-/ethanol stress-induced ulcers with an ulcer index (UI) of 50 ± 4.0/46 ± 4.0, respectively, similar to that of lansoprazole (80%) at 30 mg kg(-1) b.w. Increased H(+), K(+)-ATPase activity and thiobarbituric acid reactive substances (TBARS) were observed in ulcer-induced rats, while GRAE fed rats showed normalized levels and GRAE also normalized depleted/amplified anti-oxidant enzymes in swim stress and ethanol stress-induced animals. Gastric mucin damage was recovered up to 77% and 74% in swim stress and ethanol stress, respectively after GRAE treatment. GRAE also inhibited the growth of H. pylori with MIC of 300 ± 38 μg and also possessed reducing power, free radical scavenging ability with an IC(50) of 6.8 ± 0.4 μg mL(-1) gallic acid equivalent (GAE). DNA protection up to 90% at 0.4 μg was also observed. Toxicity studies indicated no lethal effects in rats fed up to 5 g kg(-1) b.w. Compositional analysis favored by determination of the efficacy of individual phenolic acids towards their potential ulcer-preventive ability revealed that between cinnamic (50%) and gallic (46%) phenolic acids, cinnamic acid appear to contribute to better H(+), K(+)-ATPase and Helicobacter pylori inhibitory activity, while gallic acid contributes significantly to anti-oxidant activity.
Zinger officinale 自古以来一直被用作治疗胃部不适的传统药物。本研究报道了姜科植物姜根茎水提物 (Ginger rhizome aqueous extract, GRAE) 的抗溃疡特性。GRAE 在 200mg/kg 体重时，对游泳应激/乙醇应激诱导的溃疡分别有高达 86%和 77%的保护作用，溃疡指数 (UI) 分别为 50±4.0/46±4.0，与 30mg/kg 体重的兰索拉唑 (80%) 相似。在溃疡诱导大鼠中观察到 H(+)、K(+) -ATP 酶活性和硫代巴比妥酸反应性物质 (TBARS) 增加，而给予 GRAE 的大鼠则显示出正常水平，并且 GRAE 还使游泳应激和乙醇应激诱导动物中耗尽/扩增的抗氧化酶恢复正常。GRAE 处理后，游泳应激和乙醇应激诱导的大鼠胃粘蛋白损伤分别恢复了 77%和 74%。GRAE 还抑制了 H. pylori 的生长，其 MIC 为 300±38μg，并且具有还原能力、自由基清除能力，IC50 为 6.8±0.4μg/mL 没食子酸当量 (GAE)。在 0.4μg 时还观察到高达 90%的 DNA 保护作用。毒性研究表明，在给予高达 5g/kg 体重的大鼠时没有致死作用。通过测定单个酚酸对其潜在抗溃疡能力的功效进行的成分分析表明，在肉桂酸 (50%) 和没食子酸 (46%) 酚酸之间，肉桂酸似乎对 H(+)、K(+) -ATP 酶和幽门螺杆菌抑制活性有更好的贡献，而没食子酸对抗氧化活性有显著贡献。
