Kumar Anand T N, Chung Euiheon, Raymond Scott B, van de Water Jeroen A J M, Shah Khalid, Fukumura Dai, Jain Rakesh K, Bacskai Brian J, Boas David A
Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA.
Opt Lett. 2009 Jul 1;34(13):2066-8. doi: 10.1364/ol.34.002066.
We show that fluorescence lifetime is a powerful contrast mechanism that can enhance the whole-body imaging of fluorescent proteins (FPs), in the presence of background tissue autofluorescence (AF). The nonexponential AF decay is characterized from time-domain (TD) measurements on multiple nude mice and separated from the FP fluorescence using a linear fit to a priori basis functions. We illustrate this approach using an orthotopic mouse tumor model of breast adenocarcinoma. We also report that four commonly used FPs show distinct lifetimes, indicating their suitability for in vivo lifetime multiplexing. These results suggest the potential for exploiting fluorescence lifetime for imaging FPs for a variety of whole-body small-animal imaging applications.
我们表明，在存在背景组织自发荧光（AF）的情况下，荧光寿命是一种强大的对比机制，可增强荧光蛋白（FPs）的全身成像。非指数AF衰减通过对多只裸鼠的时域（TD）测量来表征，并使用对先验基函数的线性拟合与FP荧光分离。我们使用乳腺腺癌的原位小鼠肿瘤模型说明了这种方法。我们还报告说，四种常用的FPs显示出不同的寿命，表明它们适用于体内寿命复用。这些结果表明，利用荧光寿命对FPs进行成像在各种全身小动物成像应用中具有潜力。
