Ebrahimzadeh M A, Nabavi S F, Nabavi S M
Department of Medicinal Chemistry, School of Pharmacy, Mazandaran University of Medical Sciences, Postal Code 48189, Sari, Iran.
Pak J Biol Sci. 2009 Mar 1;12(5):447-50. doi: 10.3923/pjbs.2009.447.450.
In this study antioxidant activity of methanol extract of Sambucus ebulus L. flower was investigated employing various in vitro assay systems, i.e., DPPH and nitric oxide radical scavenging, hydrogen peroxide scavenging, reducing power, iron ion chelating power and linoleic acid. IC50 for DPPH radical-scavenging activity was 228 +/- 12 microg mL(-1). The extract showed very high activity in the reducing power assay that was comparable with positive control, vitamin C. The extract showed good nitric oxide-scavenging activity (IC50 = 309 +/- 14 microg mL(-1). It was found that antioxidant activity was dose dependent i.e., activity was increased with the increase of their concentrations. The extract showed very weak activity in iron ion chelating (IC50 = 1.3 +/- 0.07 mg mL(-1)). It is showed very good activity in scavenging of hydrogen'peroxide. IC50 for scavenging of extract was 59.5 +/- 3.3 mcirog mL(-1). The extracts exhibited no activity in linoleic acid model. The total phenolic content of flower was 56.3 +/- 2.81 mg gallic acid equivalent g(-1) of extract powder and total flavonoid content was 14.5 +/- 0.72 mg quercetin equivalent g(-1) of extract powder by reference to standard curve.
在本研究中，采用多种体外分析系统，即1,1-二苯基-2-三硝基苯肼（DPPH）和一氧化氮自由基清除、过氧化氢清除、还原力、铁离子螯合能力和亚油酸，对西洋接骨木花甲醇提取物的抗氧化活性进行了研究。DPPH自由基清除活性的半数抑制浓度（IC50）为228±12微克/毫升。该提取物在还原力分析中表现出非常高的活性，与阳性对照维生素C相当。该提取物表现出良好的一氧化氮清除活性（IC50 = 309±14微克/毫升）。发现抗氧化活性呈剂量依赖性，即活性随浓度增加而增强。该提取物在铁离子螯合方面表现出非常弱的活性（IC50 = 1.3±0.07毫克/毫升）。它在过氧化氢清除方面表现出非常好的活性。提取物清除的IC50为59.5±3.3微克/毫升。该提取物在亚油酸模型中无活性。参照标准曲线，花的总酚含量为56.3±2.81毫克没食子酸当量/克提取物粉末，总黄酮含量为14.5±0.72毫克槲皮素当量/克提取物粉末。
